非小细胞肺癌组织中ETS-1和U-PA的表达及意义

Expression and Significance of ETS-1 and U-PA in Non-small Cell Lung Cancer

ETS-1(E26transformation specific-1)可以上调U-PA(urokinase-type plasminogen activator)的表达,但两者相关性的大小尚不清楚.通过采用免疫组化和RT-PCR(reverse transcriptase polymerase chain reaction)检测非小细胞肺癌(non-small cell lung cancer,NSCLC)和癌旁正常组织中ETS-1和U-PA的表达.免疫组化结果显示,50例NSCLC组织中,ETS-1和U-PA蛋白阳性率分别为70%(35/50)和64%(32/50),均显著高于对应的癌旁正常组织15%(3/20)和10%(2/20)(P<0.001).ETS-1阳性率随着肿瘤侵润深度的增加,淋巴结转移和临床分期的增加而增加(P<0.05).U-PA阳性率随着淋巴结转移和临床分期的增加而增加(P<0.05).ETS-1和U-PA蛋白表达呈正相关(r=0.059,P<0.01).在17例癌组中和癌旁组中,ETS-1、U-PA mRNA的相对表达强度分别为(0.564±0.059)和(0.318±0.089)(P=0.000)、(0.773±0.191)和(0.401±0.193)(P=0.000).结果表明,ETS-1和U-PA的过量表达与NSCLC的发展密切相关,两者的表达呈正相关,ETS-1可能通过上调U-PA的表达,促进肺癌的进展.

U-PA expression can be up-regulated by ETS-1,but the value of their relationship was not clear.The expression of ETS-1and U-PA in non-small cell lung cancer（NSCLC）and normal tissues were detected by immunohistochemistry and RT-PCR.Immunohistochemical staining showed that the expression rate of ETS-1 and U-PA protein in NSCLC tissue were 70%（35 /50） and 64%（32 /50）,which is significantly higher than that in the normal tissues,15%（3 /20）and 10%（2 /20）（P 0.001）,respectively.The expression rate of ETS-1 protein was increased in deeper of invasion,lymph node metastasis and higher clinical stages （P 0.05）of NSCLC.The expression rate of U-PA protein was increased in lymph node metastasis and higher clinical stages（P0.05）of NSCLC.A positive correlation between the expression of ETS-1 and U-PA protein was found（r=0.509,P0.01）.The relative expression intensity of ETS-1and U-PA mRNA in 17 NSCLC tissues were（0.564 ± 0.059）and（0.773 ± 0.191）,significantly higher than that in corresponding normal tissues（（0.318±0.089）（P=0.000）,and（0.401±0.193）（P=0.000））.The results indicated that the progression of NSCLC was correlated closely with over-expression of ETS-1 and U-PA.There was a positive correlation between the expression of ETS-1 and U-PA protein.ETS-1 might be involved in progression of NSCLC via up-regulating the expression of U-PA.