金针菇菌柄特异性蛋白质Flammutoxin的表达

A Specific Protein,Flammutoxin Expressed in the Flammulina velutipes Stipe

通过地高辛标记的Flammutoxin基因特异性探针与金针菇(Flammulina velutipes)菌丝体、菌柄和菌盖中的mRNA进行杂交,结果表明在金针菇的菌柄中有该蛋白质的表达,而在菌丝体和菌盖中均无该蛋白质的表达.此外在检测的平菇(Pleurotus ostreatus)、香菇(Lentinus edodes)、杏鲍菇(P.eryngii)和双孢蘑菇(Agaricus bisporus)中也无该蛋白质基因的表达.

Flammutoxin gene probe labeled by digoxigenin was hybridized with mRNA of mycelium,stipe and pileus of Flammulina velutipes,respectively.The result showed that flammutoxin was expressed in the stipe of F.velutipes,but not in its mycelium and pileus.Furthermore,no exepression of flammutoxin gene was detected in Pleurotus ostreatus,Lentinus edodes,P.eryngii,and Agaricus bisporus by the Northern blotting hybridization with the flammutoxin gene probe labeled by digoxigenin.