苍术GC指纹图谱研究

GC fingerprint chromatography of Rhizoma Atractylodis

目的:建立来自14个地区苍术的GC指纹图谱,为其质量评价提供依据。方法:苍术粉碎后经乙酸乙酯萃取制成萃取液,采用气相色谱法,HP-INNOWax Polyethylene Glycol(30.0m×0.32mm×0.5μm)石英毛细管柱,以FID为检测器,正十五醇为内标,气化室温度270℃,柱温185℃,建立苍术指纹图谱。结果:苍术指纹图谱由10个峰组成,其峰面积大于90%,其精密度、稳定性、重复性均符合指纹图谱研究的技术要求(RSD<5%)。结论:不同产地苍术在组分的分布上差异不大,但在成分的比例和量上差异较大,本法重现性好,简单,易行,可作为苍术质量控制的依据。

AIM：To establish the GC fingerprinting of Rhizoma Atractylodis collected from fourteen habitats.METHODS：Atractylodis extract was produced by extracting the crushed Rhizoma Atractylodis with ethy acetate HP-INNOWax Polyethylene Glycol（30.0 m × 0.32 mm × 0.5 μm） was used for GC column with FID detector;internal standard was 1-Pentadecanol at injection chamber temperature of 270 ℃ and column temperature of 185 ℃.RESULTS：Fingerprint of Rhizoma Atractylodis consisted of 10 peaks.The area of peaks exceeded 90% of total one.RSD of precision and reproducibility was in the range of 5%.CONCLUSION：The distribution of constituents of Rhizoma Atractylodis differ a little from different habitats,but the proportion of the constituents differs greatly.This method is reproducible,simple and easy,and can be used to provide a base for the quality control of Rhizoma Atractylodis.