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迎春樱组培无菌外植体获得方法初探 被引量:5

Preliminary Study on Sterile Explants of Cerasus Discoidea
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摘要 迎春樱种子具有休眠作用,赤霉素处理可有效打破迎春樱种子的休眠作用,促进其快速萌发;三种无菌外植体获得方法中,以光照培养箱培养30~40 d的迎春樱沙床苗作为外植体最为理想,平均诱导率为65.0%~68.3%;其次为不同浓度GA处理的MS培养基诱导形成的无菌苗,由此种方法形成的无菌苗进行芽的诱导,诱导率在90%以上,污染极少,但无菌苗诱导困难,浪费种子及培养基;当年生带腋芽茎段不适宜作为外植体进行芽的诱导,诱导率极低且后期外植体污染严重,可采用当年生带顶芽茎段进行芽的诱导;前期高浓度的BA刺激,后期适当减少培养基中BA的含量可有效诱导迎春樱丛芽的分化. The seeds of Cerasus discoidea have the dormant characteristics,and GA treatment could effectively remove the seed dormancy and promote the seed germination.Of the three methods of obtaining sterile explants,the sand bed seedling is the best way to obtian sterile explants,when the seeding growth from 30 to 40 days,and the average induction rate is from 65.0% to 68.3%.The better way is sterile seedlings which come from different concentrations of GA treatment.Inducing sterile seedlings with the method,the average induction rate is more than 90%,but the sterile seedling was difficult to induce and seeds and medium and wasted.The stem with axillary buds were not good explants,having low induction rate and high contamination rate on late growth stage.Terminal buds were good explants.Early stimulation of high concentrations of BA and late low can effectively induce the differentiation of buds.
出处 《湖北民族学院学报(自然科学版)》 CAS 2010年第3期251-255,共5页 Journal of Hubei Minzu University(Natural Science Edition)
基金 江苏省高校自然科学基金项目(06KJB180045)
关键词 迎春樱 组培 外植体 诱导 Cerasus discoidea tissue culture explants induction
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