胃癌患者腹腔游离癌细胞缝隙连接蛋白43表达的研究

Connexin 43 expression in intraperitoneal free gastric cancer cells in patients with gastric cancer

目的 探讨缝隙连接蛋白43（Cx43）在胃癌腹腔转移中的作用.方法 收集75例胃癌患者的胃癌组织、癌旁组织及腹腔冲洗液中游离胃癌细胞,应用免疫组织化学染色和免疫荧光染色检测其中Cx43的表达情况,并分析腹腔脱落胃癌细胞Cx43表达与临床病理特征的关系.结果 Cx43在胃癌及癌旁组织中的阳性表达率分别为33.3%（25/75）和100%（75/75）,差异有统计学意义（P＜0.01）.腹腔脱落胃癌细胞阳性检出率为38.6%（29/75）,脱落胃癌细胞Cx43阳性表达率为72.4%（21/29）,显著高于原发胃癌组织（P＜0.01）.腹腔脱落胃癌细胞Cx43表达与肿瘤浸润深度和组织分化程度有关（均P＜0.05）.结论 Cx43基因可能参与了胃癌腹膜转移的发生机制.

Objective To examine the association of connexin 43 （Cx43） in the intraperitoneal free gastric cancer cells and clinicopathological characteristics. Methods Immunohistochemistry and immunofluorescence staining were used to detect connexin 43 in 75 paraffin-embedded gastric cancer tissues, matched paracancerous tissue, and intraperitoneal free gastric cancer cells. Results The positive rates of Cx43 expression were 33.3%（25/75） in gastric cancer tissue specimens and 100%（75/75） in matched paracancerous tissue（P〈0.01）. The positive detection rate of free cancer cells in peritoneal lavage was 38.6%（29/75） and the positive rate of Cx43 in peritoneal free gastric cancer cells was 72.4%（21/29）, which was significantly higher than that in gastric cancer tissue specimens（P〈0.01）. Significant association was observed of Cx43 expression of free gastric cancer cells with tumor infiltration and histological type（P〈0.05）. Conclusion Cx43 gene may be involved in the mechanism of peritoneal metastasis of gastric cancer.