长期高温预处理对力竭运动后T淋巴细胞亚群的影响

Effects of long term hyperthermic pretreatment on T-lymphocyte subsets after exhaustive exercising

为研究长期高温预处理对T淋巴细胞亚群数量变化的影响,以探讨长期高温预处理能否对机体免疫机能产生影响。实验方法:10名男性受试者在一个月的高温预处理(环境温度46℃,相对湿度90%)前后采用Bruce方法进行力竭运动,并采集安静时、运动后即刻、运动后1h、运动后24h肘静脉血,测试T淋巴细胞亚群数量。实验结果:经过长期高温预处理,安静时CD4+增高且差异具有显著性(P<0.05),CD3+、CD8+增高且差异具有非常显著性(P<0.01)。运动后即刻CD3+、CD4+差异没有显著性(P>0.05),CD8+增高且差异具有显著性(P<0.05)。CD4+/CD8+值变化规律相同,差异没有显著性(P>0.05)。运动后24h各指标差异均没有显著性(P>0.05)。结果说明:经过长期高温预处理安静时机体仍处于免疫稳态,没有发生免疫功能失常,提示长期高温预处理对机体免疫有预防保护作用;运动后即刻至运动后1h,机体免疫抑制情况比高温预处理前轻。

Purposes: To study the effects of long-term hyperthermic pretreatment on changes of the quantity of T-lymphocyte subsets,and to probe into whether long term hyperthermic pretreatment produces any effects on the immune functions of the human body.Experiment method: 10 male testees did an exhaustive exercise by applying the Bruce method before and after the one-month hyperthermic treatment (ambient temperature: 46oC,relative humidity: 90%);their elbow venous blood was drowned and their quantity of T-lymphocyte subsets was tested in a calm condition,immediately after exercising,1h after exercising,and 24h after exercising.Experiment results: after long term hyperthermic pretreatment,in a calm condition,CD4+ increased and the difference was significant (P<0.05),while CD3+ and CD8+ increased and the differences were very significant (P<0.01);immediately after exercising,the differences in CD3+ and CD4+ were not significant (P>0.05),while CD8+ increased and the difference was significant (P<0.05);the patterns of changing of CD4+ and CD8+ values were identical,and the differences were not significant (P>0.05);24h after exercising,the differences in various indexes were all not significant (P>0.05).Result suggestions: after long term hyperthermic pretreatment,in a calm condition,the human body was still in an immunization stable state,having no immune function disorders,which suggests that long term hyperthermic pretreatment plays a role in preventive protection of immunization of the human body;from immediately after exercising to 1h after exercising,the condition of immunosuppression of the human body was less serious than that before hyperthermic pretreatment.