脑脊液寡克隆区带琼脂糖凝胶免疫固定电泳的实验研究

The Study of Cerebrospinal Fluid Oligoclonal Bands Using Electrophoresis Method

目的鉴定脑脊液寡克隆区带(oligoclonal band,OB)的存在,探讨其在中枢神经系统疾病中的临床应用。方法对脑脊液和血清标本同时进行琼脂糖凝胶电泳,使用酶标抗人免疫球蛋白G,采用免疫固定电泳技术后,用TTF3显色。鉴定是否存在寡克隆区带。结果 67例神经系统疾病患者,其中4例临床确诊为多发性硬化症(multiple sclerosis,MS),经电泳免疫固定酶标显色后,2例(50%)发现寡克隆蛋白阳性,其他为阴性。结论脑脊液免疫固定电泳是检测脑脊液中内源性合成免疫球蛋白的直观方法。OB的检查对于多发性硬化和神经系统炎症有一定的诊断价值。

Objective To study the method for the detection of cerebrospinal fluid （CSF） oligoclonal bands （OB） and to investigate the clinical application in neurological diseases.Methods Cerebrospinal fluid （CSF） and serum were separated by agarose gel electrophosis, probed with antibody IgG （Anti-IgG）, and stained with TTF3.The oligoclonal bands were displayed in positive sample.Results 67 samples with neurological diseases were studied （34 male, 33 female, 2-71 years old）.There were tow positive in four multiple sclerosis （MS） patients and none for the other neurological diseases patients.Conclusion Immunefixation electrophoresis is a direct method in examination of endogenous IgG of CSF.Detection of the OB was useful in the diagnosis of MS.This study successfully set up the method for the detection of OB in CSF.