活动期类风湿关节炎患者外周血树突状细胞CCR5和CCR7的检测及意义

Detection and its clinical value of CCR5 and CCR7 in dendritic cells from patients with active rheumatoid arthritis

目的观察活动性类风湿关节炎(RA)患者不同活动期树突状细胞(DCs)表面趋化因子受体CCR5和CCR7的表达,评价其与疾病活动指标的相关关系。方法选取28名RA患者以DAS28评分分为:低活动组、中活动组和高活动组,10名正常健康人为正常组,体外分离和培养RA患者DCs。采用流式细胞仪检测RA患者DCs表面CCR5和CCR7的表达。采用直线相关分析CCR5、CCR7和RA患者类风湿因子(RF)、C反应蛋白(CRP)与抗CCP抗体的相关关系。结果 DCs表面CCR5、CCR7在RA不同疾病活动性中有差异表达,CCR5和CCR7的表达与RF和CRP有直线相关关系,与抗CCP抗体无显著相关。结论 RA患者外周DCs表面CCR5和CCR7的表达与RA疾病活动有相关关系,可能成为监测RA疾病活动和治疗效果的指标。

Objective To detect the expressions of CCR5 and CCR7 on dendritic cells（DCs） in patients with rheumatoid arthritis（RA） in different phases of disease activity,and explore the relationship between the disease activity and the expression of chemokine receptors.Methods Twenty-eight patients with low,moderate and high disease activity and 10 normal control subjects were enrolled in this study.Peripheral blood was obtained from the subjects and the DCs were isolated.The expression of CCR5 and CCR7 on DCs were detected by flow cytometry,and the serum levels of rheumatoid factor（RF）,C-reactive protein（CRP） and anti-CCP antibody（ACPA） were assessed.The correlation of the expressions of CCR5 and CCR7 to serum RF,CRP,and ACPA levels of the RA patients were analyzed.Results Compared to the normal control group,RA patients showed enhanced expressions of CCR5 and CCR7 on the DCs.A linear correlation was noted between CCR5 and CCR7 expressions on the DCs and the serum levels of RF and CRP,but not ACPA,in the RA patients.Conclusion The expressions of CCR5 and CCR7 on the DCs may correlate to the disease activity of RA,and may serve as valuable indices in monitoring the disease activity and the efficacy of the treatment.