不同途径大鼠骨髓基质干细胞同种异体肝脏移植效果的比较

Effect of different approaches to transplanting bone marrow stromal stem cells into the allogenic rat liver

目的比较不同移植途径大鼠骨髓基质干细胞(BMSCs)向同种异体大鼠肝脏移植的效果。方法选用SD大鼠30只,随机分成3组(肝内移植组、门静脉移植组、股静脉移植组),每组10只,取浓度为1×106/ml feridex标记的BMSCs1ml,分别经大鼠肝实质、门静脉、股静脉3种途径移植到大鼠体内,于移植后12h、1d、3d、5d、7d、14d用MRI分别对三组大鼠肝脏进行扫描,并于移植后第14天普鲁士蓝染色光镜观察大鼠肝脏组织切片。结果肝内移植组所有大鼠在移植后各个时间点MRI均能检测到肝脏细胞移植部位椭圆形低信号区,低信号范围随着时间的延长逐渐缩小,信号强度逐渐升高;门静脉移植组所有大鼠在移植后各个时间点MRI均能检测到肝门区门静脉内分支状低信号改变,信号强度低于肝内移植组,随着时间的延长低信号分布形态无明显变化,信号强度逐渐升高;股静脉移植组在各个时间点均未检测到明显低信号改变。移植后14d三组大鼠肝脏组织切片均可见含蓝色颗粒的细胞,肝内移植组最多,门静脉移植组次之,股静脉移植组最少,差异有显著统计学意义(P<0.05)。结论股静脉、门静脉与肝内移植三种途径的比较,肝内移植途径效果最好。

Objective To compare the effect of different approaches of bone marrow stromal stem cell（BMSCs） transplantation into the allogenic rat liver.Methods Thirty male SD rats were randomized equally into local livergroup,portal vein group,and femoral vein group,and received injection of 1×106/ml BMSCs directly into the rat liver,through the portal vein and through the femoral vein,respectively.The rat livers were scanned by magnetic resonance imaging（MRI） at 12 h and 1,3,5,7,14 days afterthe cell transplantation.Prussian blue staining of the rat liversections was also performed 14 days after the transplantation.Results MRI showed decreased signal intensity in all the rat livers of the local liver group;the ovoid area of the signal intensity gradually shrunk and the signal intensity increased with the passage of time.Lowered signal intensity was also seen in the rat livers of the portal vein group,appearing constantly branch-shaped,indistinct and increased gradually.Decreased signal intensity did not occurin the livers of femoral vein group.Prussian blue staining of all the rat livers at day 14 showed the presence of cells containing blue particles in all the groups,most numerous in the local livergroup followed by the portal vein group and then the femoral vein group.Conclusion Direct intrahepatic injection of the BMSCs results in better effect than cell transplantation via the portal vein orthe femoral vein.