Rh血型抗-D阳性个体中未见DEL型

Rh blood group DEL types are not found among anti-D positive individuals

目的分析Rh血型系统抗-D抗体阳性个体的RhCcEe表型及检测DEL等位基因,观察产生抗-D同种免疫反应个体中是否存在DEL型。方法对因输血或妊娠产生抗-D同种抗体的个体,采用间接抗人球蛋白试验(IAT)排除部分D型或弱D型,然后进行RhCcEe表型检测,同时采用PCR方法检测RHD1227A等位基因鉴别DEL型和真实Rh(D)阴性表型。同时检测99名IAT确认的、抗-D抗体阴性的Rh(D)阴性健康人作对照。结果 99名抗-D抗体阴性组共检出C抗原阳性(包括CC或Cc)个体44名(44.4%);DEL型24名(24.2%)。抗-D抗体阳性组经IAT确认共118名Rh(D)阴性,C抗原阳性22名(18.6%),未发现DEL型个体,均与抗体阴性组存在显著性差异(P<0.01)。结论抗-D抗体阳性组未见DEL型,提示DEL型个体或不会因输血或妊娠针对Rh(D)抗原发生抗-D同种免疫反应;由于DEL型多携带C抗原,因此造成抗-D阳性组C抗原频率显著降低。

To discuss if DEL could be immunized by D antigen clinically,both the Rh blood group DCcEe antigen phenotypes and DEL alleles were determined in anti-D positive individuals.We detected CcEe phenotypes for the allo-anti-D positive individuals due to pregnancies or transfusions.The D antigen was identified by the IAT to exclude partial D or weak D phenotypes.The RHD1227A DEL alleles were tested through a PCR test.As a control,we also detected 99 IAT-negative apparent Rh（D） negative donors.Among 99 Rh（D） negative donors,44 C-positive（including CC and Cc phenotypes） were detected（44.4%）,and 24 DEL types were found（24.2%）.Total 118 Rh（D） negative individuals with anti-D positive were observed.Among them,22 C-positive individuals were found with a rate of 18.6%,but no DEL detected,which showed a significant different with anti-D negative group（P 0.01）.In conclusion,no DEL was observed in the anti-D positive group,which shows that the DEL variant is not at risk of alloimmunization to the D antigen through pregnancies or transfusions,and reduce the C-positive rate significantly in the anti-D positive group.