三叶因子2在大鼠下颌下腺内的表达及其与实验性胃溃疡愈合的关系

Relationship between the expression of trefoil factor 2 in rat submaxillary glands and the self healing of experimental gastric ulcer

目的探讨大鼠实验性胃溃疡自愈期间三叶因子2(TFF2)在大鼠下颌下腺的表达及其与胃溃疡愈合的关系。方法 48只雄性SD大鼠分为溃疡组和正常组,采用免疫组织化学和RT-PCR法,检测溃疡组和正常组大鼠下颌下腺中TFF2肽和基因的表达情况。结果 TFF2免疫反应阳性物质主要位于纹状管、闰管和小叶间导管、颗粒曲管少颗粒细胞的胞质,管腔内亦有阳性物质表达,近腔面处较多。和正常组相比,溃疡1d时TFF2阳性物质面密度和积分吸光度明显增高,2d时最低,4、6d时逐渐升高(P<0.01),10～23d时均维持在较高水平(P<0.05)。溃疡1、2、4、6、10、14、23d TFF2/GAPDH吸光度比值除2d溃疡组略有增高外,其他各溃疡组明显高于正常对照组(P<0.01或P<0.05)。结论大鼠下颌下腺中TFF2参与了胃溃疡愈合过程的调节。

Objective To explore the expression of trefoil factor 2（TFF2） in submaxillary glands during the self healing of experimental gastric ulcer in rats. Methods Totally 48 male SD rats were randomly divided into gastric ulcer group（ n = 42）and normal group （n = 6）. The expression of TFF2 peptide and TFF2 gene were detected by the methods of immunohistochemistry and RT-PCR respectively in the gastric ulcer group and normal group. Results TFF2 immunoreactive cells were mainly located in the cytoplasm of the epithelial cells in the ducts, such as intercalated duct, striated ducts, interlobular duct, and which also showed positive expression in the cavity,even near the cavosurface. TFF2 peptide also showed strong expression in few granule cells or in those cells without granules, and which were weakly positive or negative in the cells with many granules. The integral absorbance values （IA） and area density （AD） values obviously increased on the day 1 after gastric ulcer（ P 〈 0. 01 ） , and which was the lowest on the day 2 （ P 〉 0.05 ） , then gradually increased again on the day 4 and day 6 （P 〈 0.01 ） , and kept on a high level till 10 days to 23 days compared with the normal group（ P 〈 0.05 ）. The IA of TFF2/GAPDH in 1,4,6,10,14 and 23 days gastric ulcer group were obviously higher than that of the normal group except the 2-day gastric ulcer group （ P 〈 0.01 or P 〈 0.05 ）. Conclusion TFF2 in rat submaxillary glands may participate in the regulation of self healing in gastric ulcer.