小鼠发育期卵巢成纤维细胞生长因子10的表达

Expression of fibroblast growth factor 10 during the development of mouse ovaries

目的研究小鼠卵巢发育期成纤维细胞生长因子10(FGF10)的表达。方法分离孕E14.5、E16.5、E18.5、生后第1天和3周龄小鼠卵巢,每组实验动物6只,应用免疫组织化学技术研究FGF10蛋白在卵巢中的表达;3周龄小鼠腹腔注射孕马血清促性腺激素(PMSG)48h后,再注射人绒毛膜促性腺激素(hCG),hCG注射后3h提取总mRNA,应用RT-PCR技术研究卵巢中FGF10、黄体生成素受体(LHR)、成纤维细胞生长因子7(FGF7)和成纤维细胞生长因子受体2Ⅲb(FGFR2Ⅲb)基因的表达变化。结果 FGF10蛋白表达于卵母细胞,并且FGF10在生后第1天小鼠的卵母细胞上表达最强;两种促性腺激素处理后,FGF10 mRNA与对照组相比表达下降,LHR mRNA表达升高,FGF7和FGFR2Ⅲb mRNA表达不变。结论 FGF10蛋白特异表达于小鼠卵母细胞膜,促性腺激素影响卵巢FGF10 mRNA的表达,推测FGF10可能参与调控小鼠卵母细胞的生长和卵泡发育。

Objective To examine the expression of fibroblast growth factor 10 （FGFIO） during the development of mouse ovaries. Methods Ovaries were isolated from mice at gestation dayl4.5, dayl6.5, dayl8.5, newborn and three-week-old. The samples of each group were six. FGF10 protein was detected in mice ovaries by imnmnohistoehemistry. Three-week-old mice were treated with pregnant mare serum gonadotrophin （PMSG）, by intraperitoneal injection and forty-eight hours after human chorionic gonadotrophin （ hCG ） was injected into those mice, mRNA was extracted after three hours of injection hCG, the expression of FGF10, luteinizing hormone receptor（LHR） , FGF7 and fibroblast growth factor receptor 2 Ⅲ b （FGFR2 Ⅲ b） mRNA in ovaries were compared by RT-PCR. Results FGF10 protein expressed in the oocytes in different developmental stages of mouse ovary, and the highest level of staining was observed in the oocytes of the newborn mouse. After gonadotrophins injection, RT-PCR results showed that the expression of FGF10 mRNA decreased, but LHR increased, and FGF7 and FGFR2 Ⅲ b mRNAs levels unchanged. Conclusion FGF10 is localized on the cell membrane of oocytes of mouse, and gonadotrophins affect the expression of FGFIO mRNA in the ovary of mouse. These results indicate that FGF10 may be connected with regulating ooeytes growth and follicle development of mouse.