Ⅲ型β-微管蛋白和多药耐药基因1蛋白表达与非小细胞肺癌生物学特性的相关性

Correlation of the expression of III β-tubulin and MDR1 protein with biological features of non-small cell lung cancer

目的 探讨Ⅲ型β-微管蛋白（Ⅲβ-tubulin）和多药耐药基因1（MDR1）蛋白在非小细胞肺癌（NSCLC）中的表达特征及临床意义.方法 应用免疫组化法检测158例NSCLC组织中Ⅲβ-tubulin和MDR1蛋白的表达,应用Western blot检测新鲜NSCLC组织及相应癌旁正常组织中Ⅲβ-tubulin和MDR1蛋白的表达,并分析其表达与NSCLC生物学特性的关系.结果 Ⅲβ-tubulin和MDR1蛋白在NSCLC中的阳性表达率分别为65.2%和51.3%.Western blot结果显示,Ⅲβ-tubulin和MDR1蛋白在NSCLC组织中的表达明显高于癌旁正常肺组织（P＜0.01）.Ⅲβ-tubulin在中、高分化腺癌中的表达明显低于低分化腺癌（P＜0.01）,肺腺癌中MDR1蛋白的表达明显高于鳞癌和大细胞未分化癌（P＜0.01）.Ⅲβ-tubulin在Ⅲ～Ⅳ期NSCLC中的表达高于Ⅰ～Ⅱ期（P＜0.05）,而MDR1蛋白的表达差异无统计学意义（P＞0.05）.Ⅲβ-tubulin和MDR1蛋白阳性表达与患者年龄、性别、肿瘤大小及淋巴结状态无明显相关性（P＞0.05）.结论 Ⅲβ-tubulin和MDR1蛋白在NSCLC的发生、发展中有一定作用,可作为判断NSCLC预后的重要指标.

Objective To explore the expression of Ⅲ β-tubulin and MDR1 protein in patients with non-small cell lung cancer （NSCLC）, and to clarify its clinical significance. Methods Paraffin embedded tissues from 158 primary non-small cell lung cancers and para-cancerous lung tissues were investigated for the expression of Ⅲ β-tubulin and MDR1 protein by immunohistochemistry, as well as in freshly-taken NSCLC tissues by Western blot. The relationship between the expression of Ⅲ β-tubulin and MDR1 and the biological features of lung cancer was analyzed. Results The positive rate of Ⅲ β-tubulin and MDR1 protein expression in lung cancer tissues was 65.19% and 51.27%, respectively. Western blot analysis showed that the level of of Ⅲ β-tubulin and MDR1 protein in NSCLC tissues was remarkably higher than that in normal tissues （ P 〈0.01）. The expression of Ⅲ β-tubulin in stage Ⅲ-Ⅳ cases was significantly higher than that in stage Ⅰ - Ⅱ cases （ P 〈 0.05 ）, while the expression of MDR1 protein showed no significant difference （P 〉 0.05）. The positive rate of Ⅲ β-tubulin expression in well-moderate pathological grades was lower than that in poor ones. The positive rate of MDR1 expression in adenocarcinoma was higher than that in squamous cell carcinoma and large cell undifferentiated cancers （ P 〈 0. 01 ）. The positive rate of expression of MDR1 protein and Ⅲ β-tubulin was not correlated with sex, age, tumor size and lymph node metastasis （P 〉 0.05 ）. Conclusion The expression of Ⅲ β-tubulin and MDR1 may play an important role in the development and progression of human non-small cell lung cancer, and could be looked as an important index for judging the prognosis of lung cancer.