丹酚酸B与甘草酸对大鼠门静脉灌流压力的影响

Effects of Salvianolic Acid B and Diammonium Glycyrrhizinate on Portal Pressure in Rats

目的观察甘草酸与丹酚酸B对离体大鼠门静脉的舒缩作用。方法 Wistar大鼠肝动脉,门静脉和肝静脉插管。经肝动脉冲净肝内存血液,灌流状态下游离肝脏;苯肾上腺素预收缩恒流门脉,生物机能实验系统观察甘草酸与丹酚酸B对门静脉灌流压的影响,Prism-4非线型可变斜率回归计算半效浓度。结果甘草酸对苯肾上腺素收缩门脉舒张作用的半效浓度1.5556×10-9mol/L,提示其直接舒张作用为治疗门脉高压的药理机制之一。丹酚酸B对苯肾上腺素收缩门脉具有收缩作用,半效浓度为1.4639×10-9mol/L;提示其间接调控作用参与对门脉高压的协同治疗。结论在恒流测压模式下,甘草酸对离体门脉具有舒张作用,而丹酚酸B具有收缩作用。

Objective To study the relaxant effects of glycyrrhizinate and salvianolic acid B on rat portal vein in vitro.Methods Healthy female Wistar rats were canalized from hepatic artery,portal vein and hepatic vein in vitro.Remained blood in liver was eliminated with heparinized Krebs-Henseleit solution through hepatic artery,then the liver was isolated under infusing manner.Being constricted with phenylephrine and relaxed with acetylcholine,and infused with glycyrrhizinate or salvianolic acid B,the portal pressures of infused rat livers were consistently monitored by BL-420S physiological experiment system.The median effective concentration（EC50）of the two agents were analyzed with non-linear various slope regression using Prism-4 software.Results EC50 of glycyrrhizinate in relaxing the phenylephrine-contracted portal was 1.5556×10-9mol/L,suggesting one of the mechanism of action of diammonium glycyrhizinate for the treatment of portal hypertension was direct relaxation.Salvianolic acid B showed constrictive action on the phenylephrine-retracted portal vein,the EC50 was 1.4639×10-9mol/L,indicating that its indirect control action was took part in the portal hypertension therapy synergistically.Conclusion Under the mode with both controlized-velocity and monitored pressure,glycyrrhizinate showed relaxation and salvianolic acid B showed constriction on portal pressure in vitro.