中药清肠栓对溃疡性结肠炎大鼠结肠通透性的影响

Effect of Qingchang Suppository on Intestinal Permeability in Rats with Ulcerative Colitis

目的探讨复方中药清肠栓对三硝基苯磺酸(TNBS)诱导的溃疡性结肠炎(UC)大鼠结肠黏膜通透性的影响及其修复结肠溃疡的作用机制。方法采用异硫氰酸荧光素-右旋糖4000(FITC-dextran4000,FD-4)作为标记物,在体实验与离体实验相结合测定大鼠结肠通透性,观察中药清肠栓对UC大鼠结肠通透性的影响。结果 (1)在体动物实验:发现模型组大鼠结肠对FD-4的通透性较正常组显著增加,在30min内增加为对照组的6倍。清肠栓高剂量及中剂量组能不同程度的抑制UC大鼠结肠通透性的升高(P<0.05)。(2)离体实验:通过测定FD-4的通透性系数(Papp)反映发现,大鼠升结肠黏膜通透性120min内,模型组FD-4的Papp为(5.001±1.316)×10-8cm/s,Papp增加为正常组的2.5倍,清肠栓高剂量及中剂量组能有效降低升结肠黏膜对FD-4的Papp(P<0.05)。结论清肠栓能有效抑制UC大鼠结肠通透性的升高,改善肠黏膜屏障功能,促进溃疡愈合。

Objective To observe the effect of Qingchang Suppository（QCS,a Chinese herbal preparation）on intestinal permeability in rat ulcerative colitis（UC）model induced by trinitrobenzene sulforic acid,and to explore the mechanism of QCS for healing the ulceration.Methods Labelled by FITC-dextran 4 000（FD-4）,the permeability of colonic membrane in UC rat and effect of QCS on it were observed in vitro and in vivo.Results In vivo study showed that the colonic FD-4 permeability of UC rat was increased significantly,being 6-fold of normal in 30 min.After treated with QCS of high/moderate dosage,it significantly attenuated to different degrees（P0.05）.FD-4 permeability coefficient（Papp）determination in vitro showed that Papp in model rats increased to（5.001± 1.316）×10-8cm/s in 120 min,being 2.5-fold of control;and which could be decreased by high/moderate dose QCS effectively（P0.05）.Conclusion QCS could suppress the high colonic permeability in UC model rats,improve the barrier function of intestinal membrane and promote the healing of ulceration.