尿道下裂患儿包皮组织中FGFR2表达检测及意义

FGFR2 expression in preputial tissue of hypospadias

目的 检测成纤维细胞生长因子受体2（fibroblast growth factor receptor 2,FGFR2）蛋白和mRNA在包皮环切组和尿道下裂组包皮组织中的表达情况,初步探究FGFR2蛋白表达、基因功能和尿道下裂的关系.方法 采用Western blotting（免疫印迹）方法和RT-PCR方法;检测两组中FGFR2表达情况;结果包皮环切组和尿道下裂组FGFR2蛋白相对定量值分别为0.39±0.12和0.19±0.09（P＜0.05）,尿道下裂组较包皮环切组降低.尿道下裂轻、中、重度三组间FGFR2蛋白相对定量值分别为0.27±0.08、0.16±0.04、0.09±0.03;中度和重度之间比较无统计学差异（P＞0.05）.包皮环切组和尿道下裂组FGFR2 mRNA表达量分别为1.30±0.069和1.22±0.052,包皮环切组高于尿道下裂组（P＜0.05）.尿道下裂轻、中、重度三组间FGFR2 mRNA定量值分别为1.23±0）.054、1.24±0.034、1.16±0.020;轻度和中度之间之间无统计学差异（P＞0.05）,重度尿道下裂FGFR2mRNA表达量低于其他两组.结论 尿道下裂患儿包皮组织中FGFR2蛋白和mRNA较包皮环切组表达下调.提示FGFR2和尿道下裂关系密切,进一步研究FGFR2蛋白和基因功能有助于明确FGFR2在尿道下裂发病机制中的重要地位,有可能揭示尿道下裂的发病机制.

Objective To assay the expression of fibroblast growth factor receptor 2（FGFR2）protein and mRNA in the preputial tissue of hypospadias patients. Methods Western blotting and R-TPCR were performed to assay the expression of FGFR2 protein and mRNA. Results The amount of FGFR2 protein in the preputial tissue of hypospadias patients was lower than the control group（0. 19± 0. 09 vs 0. 39 ± 0. 12, P＜0. 05）. The FGFR2 protein quantity was 0. 27 ± 0. 08 in the mild group,0. 16 ± 0. 04 in the moderate group and 0. 09 ± 0. 03 in the severe group. The difference between hypospadias group and the control group was statistically significant （P＜0. 05）. There was no difference between the moderate and severe groups（P＞0. 05）. The difference between the FGFR2 mRNA expression of the control （1.30 ± 0. 069） and hypospadias （ 1.22 ± 0. 052） groups was significant（P＜0. 05）. FGFR2 mRNA quantity was 1.23 ± 0. 0541 in the mild group, 1.24 ± 0. 034 in the moderate group, 1.16 ± 0. 020 in the severe group. There was no difference between the mild and moderate groups in FGFR2 mRNA expression （P＞0. 05）. Conclusions The expression of FGFR2 of preputial tissue in hypospadias was down-regulated. It suggestes a role for FGFR2 in hypospadias