摘要
前文报道环境诱变剂丝裂霉素Ｃ（ＭＭＣ）、９氨基吖啶（９ＡＡ）和甲基磺酸乙酯（ＥＭＳ）对家蚕核型多角体病毒（ＢｍＮＰＶ）多角体形态有明显的诱变作用。本文进一步报道形态变异的ＢｍＮＰＶ多角体在继代实验中的仍保持相对稳定的比率［（２１３±４２８）％～（３００±４８５）％］，说明诱变的异常多角体能较稳定地遗传到次代，ＭＭＣ及９ＡＡ诱变的ＢｍＮＰＶＤＮＡ，经ＥｃｏＲⅠ、ＢａｍＨⅠ和ＢｇｌⅡ酶切的电泳图谱与野生型对照株相比较，出现某些ＤＮＡ泳带的增加或丢失。
In our early studies,Mitomycine (MMC),Aminoacridine (9 AA) and Ethyl methanesulfnonate (EMS) were used as environmental mutagens to Bombyx mori nuclear polyhedrosis virus (BmNPV).They distinctly induced the morphological change of polyhedra. The present studies showed that the variant polyhedra had relative stability in the next generation with high rate [about (21.3±4.28)%～(30.0±4.85)%].The BmNPV DNA of MMC and 9 AA mutants were digested by EcoR Ⅰ,BamH Ⅰ and Bgl Ⅱ,and some additional DNA bands appeared and some disappeared on the electropherogram as compared with the control of the BmNPV DNA.This shows that mutation of BmNPV genome had occurred at some hotspots.
出处
《蚕业科学》
CAS
CSCD
1999年第1期31-35,共5页
ACTA SERICOLOGICA SINICA
基金
广东省自然科学基金
