摘要
应用三维荧光结合二阶校正方法对中药厚朴及平胃片中的主要成分厚朴酚、和厚朴酚进行了定量分析研究.实验对影响荧光强度的主要因素进行了考察,确定用pH3的乙酸/乙酸钠缓冲溶液控制实验酸度,用浓度为7.0×10-2mol.L-1的表面活性剂SDS增加荧光灵敏度.二阶校正算法AATLD解析得到的厚朴酚与和厚朴酚的加样平均回收率分别为(95.67±1.75)%和(92.45±1.52)%.
In the presence of interfering components and the simple performance condition,a method is proposed for simultaneous determination of magnolol and honokiol by combining excitation emission fluorescence spectrum with second-order calibration algorithm.The average recovery of magnolol and honokiol is（95.67±1.75）% and（92.45±1.52）% respectively for AATLD algorithm.It shows that the second-order calibration methods can quantify the analysis of interest from overlapped chromatographic profiles and give the accurate predicted results by utilizing ＂mathematical separation＂ instead of ＂physics separation＂.
出处
《平顶山学院学报》
2010年第5期53-57,共5页
Journal of Pingdingshan University
基金
河南教育厅科研基金资助项目(2008A150009)
河南工业大学博士基金(2006BS010)
湖南大学化学
生物传感与化学计量学国家重点实验室开放基金(2007009)
关键词
三维荧光
二阶校正
厚朴酚
和厚朴酚
excitation emission fluorescence
second-order calibration
magnolol
honokiol
