摘要
目的检测正常妊娠和子痫前期孕妇在中期妊娠时血清可溶性血管内皮生长因子受体1(sFlt-1)、胎盘生长因子(PLGF)水平,探讨母血sFlt-1/PLGF比值是否可作为预测孕妇发生子痫前期的标志物。方法用酶联免疫吸附试验(ELISA)检测200例正常妊娠妇女、57例子痫前期孕妇妊娠中期血清PLGF和sFlt-1水平,计算sFlt-1/PLGF比值。结果子痫前期组血清sFlt-1浓度[(1 385.0±178.8)ng/L]明显高于正常妊娠组[(1 087.3±122.7)ng/L](P<0.01),血清PLGF浓度[(501.9±86.3)ng/L]明显低于正常妊娠组[(712.7±103.6)ng/L],(P<0.01);子痫前期组sFlt-1和PLGF水平呈负相关(r=-0.629,P<0.01),正常妊娠组sFlt-1和PLGF水平呈正相关(r=0.682,P<0.01)。在妊娠中期,sFlt-1/PLGF比值>1.9时,预测子痫前期的敏感性为89.5%,特异性为97.5%。结论妊娠中期血清sFlt-1水平增高伴有PLGF水平降低与子痫前期的发病密切相关,sFlt-1/PLGF比值可以作为预测子痫前期的标志物。
Objective To detect the levels of serum soluble fms-like tyrosine kinase-1（sFlt-1） and placenta growth factor（PLGF） during the second trimester in the serum of women in normal pregnancy and women who subsequently develop preeclampsia,and investigate the role of sFlt-1/PLGF ratio as an early marker for the development of preeclampsia.Methods The serum levels of PLGF and sFlt-1 in 200 healthy pregnant women and 57 women with preeclampsia were determined by enzyme-linked immunosorbent assay（ELISA）.The ratio of sFlt-1/PLGF was calculated.Results The serum sFlt-1 levels were significantly higher in the women with preeclampsia [（1 385.0±178.8）ng/L] than in healthy controls [（1 087.3±122.7）ng/L]（P0.01）.The PLGF levels were significantly lower in the women with preeclampsia [（501.9±86.3）ng/L] than in healthy controls [（712.7±103.6）ng/L]（P0.01）.In the women with preeclampsia,sFlt-1 levels were negatively correlated with PLGF（r =-0.629,P0.01）,and in healthy controls,there were positively correlations between the PLGF and sFlt-1 levels（r =0.682,P0.01）.In the second trimester,the ratio of sFlt-1/PLGF was 1.9,and its sensitivity and specificity were 89.5% and 97.5%.Conclusions Preeclampsia showes a strong association with increased levels of sFlt-1 and decreased levels of PLGF in the second trimester.The sFlt-1/PLGF ratio may be an early marker for the development of preeclampsia.
出处
《检验医学》
CAS
北大核心
2010年第10期784-786,共3页
Laboratory Medicine
基金
江苏省常州市科技规划项目(CS2008224)
