不同血液分析仪测定机采血小板计数结果的比较分析

The comparison and analysis of the apheresis platelet count results with different blood analyzers

目的观察不同血液分析仪测定机采献血者采前与成品标本中血小板(PLT)数量的准确性。方法先对绍兴市中心血站2台仪器(三分类分析仪及五分类分析仪)计数PLT的批内、批间精密度进行测定,再对68例采前静脉血标本和52例机采后样管标本分别用绍兴市中心血站2台仪器与手工镜检法进行PLT计数,并取其中的各20例标本用其他品牌仪器进行比对,对数据进行统计分析。结果绍兴市中心血站2台仪器计数PLT均有较好的批内、批间精密度;在采前静脉血标本检测上,不同仪器间,仪器与手工镜检间比较,差异无统计学意义(P>0.05);在采后样管检测上,三分类分析仪与五分类分析仪、手工镜检间比较,差异有统计学意义(P<0.01)。结论采后成品PLT数量检测应该用五分类血液分析仪进行计数,如用三分类血液分析仪计数,则需特别注意测定值低于实际值的现象,可通过每月一次与五分类仪器或手工法比对的方法 ,调整检测系数或增大稀释倍数加以克服。

Objective To observe the accuracy of the platelet（PLT） count with different blood analyzers before and after apheresis platelet.Methods The within-run and between-run precisions of the two blood analyzers（3-classification and 5-classification blood analyzers from the Blood Center of Shaoxing） were determined.68 venous blood samples and 52 samples after apheresis platelet were determined for PLT count by the two blood analyzers and microscopic method,and 20 of those venous blood samples and 20 of those samples after apheresis platelet were performed as controls respectively which were determined by other blood analyzers.The results were analyzed statistically.Results The within-run and between-run precisions of the two blood analyzers were good.There was no significant difference among the 3-classification and 5-classification blood analyzers and the microscopic method as the venous blood was tested（P0.05）.The testing results of the samples after apheresis platelet showed that there were significant difference among the 3-classification blood analyzer,the 5-classification blood analyzer and the microscopic method（P0.01）.Conclusions The PLT count after apheresis platelet should be determined by the 5-classification blood analyzer.If it is determined by the 3-classification blood analyzer,the testing value may be lower than that of actual value,which can be adjusted the testing coefficient and dilution by comparing with the 5-classification blood analyzer and the microscopic method monthly.