青蒿琥酯对H_(22)小鼠肝癌的体内抑瘤作用

Inhibitory Effect of Artesunate on Hepatocarcinoma in H_(22) mice

目的:探讨青蒿琥酯对H22小鼠肝癌的体内抑瘤作用。方法:取雌性KM小鼠40只,将H22小鼠肝癌细胞接种于小鼠右前肢腋皮下,随机分为模型对照组(Control)、环磷酰胺组(CTX)及青蒿琥酯(ASUN)30、90、150mg/kg低、中、高三个剂量组,共5组,每组8只。连续腹腔注射给药8天后,剥取瘤块和脾脏,称重,计算抑瘤率。结果:青蒿琥酯30、90、150mg/kg剂量组的抑瘤率分别为38.9%、31.1%和21.5%,抑瘤率随剂量增大而降低,其中青蒿琥酯30mg/kg剂量组与模型对照组比较,差异有统计学意义(P<0.05);荷瘤小鼠脾质量随青蒿琥酯剂量增加而增大。结论:青蒿琥酯对H22小鼠肝癌具有体内抑瘤作用。

Objective：To analyzed the inhibitory effect of Artesunate（ASUN） on hepatocarcinoma in H22 mice.Methods：Hepatocarcinoma model was established in 40 female KM mice by implanting H22 cell line.These mice were randomized into control group,cyclophosphamide（CTX） group and ASUN group.The ASUN groups were divided into 3 subgroups based on different doses of 30,90 and 150 mg/kg.Eight mice were in each group.Intervention methods were given for 8 days and inhibitory rate was calculated after treatment.Results：The inhibitory rates were 38.9%,31.1%,and 21.5% in 30,90,and 150 mg/kg ASUN groups.The inhibitory rate in the ASUN group of 30 mg/kg was significantly different to the control group（P〈0.05）.Spleen weight bearing carcinoma increased as the dose of ASUN increased.Conclusion：ASUN can inhibit the growth of hepatocarcinoma cell in H22 mice.