摘要
以厚皮甜瓜WT-1的子叶为外植体,对离体培养和植株再生进行了研究。结果表明:4 d苗龄的子叶诱导不定芽效果最佳;子叶近轴端不定芽诱导率为96.7%,明显高于远轴端;不同激素配比中添加6-BA 1.0 mg/L的MS培养基诱导率高达91.7%,转接MS培养基上进行再生芽的伸长培养后,在1/2MS+IAA 1.0 mg/L的培养基中诱导生根,试验建立了甜瓜遗传转化和快繁的子叶再生体系。
The plantlet regengeration from cotyledon of WT-1 was studied.The results showed that 4 days old seedlings cotyledon for inducing buds was best;The rate of inducing near shaft cotyledon arrived at 96.7%,which was significantly higher than the far shaft;The most suitable medium for bud induction from cotyledon of melon was MS＋6-BA 1.0 mg/L and the rate of inducement arrived at 91.7%.Buds elongated at MS.Then the rooting media were 1/2MS＋IAA 1.0 mg/L.The system of genetic transformation and quick propagation for cotyledon of watermelon was established preliminarily.
出处
《北方园艺》
CAS
北大核心
2010年第20期133-135,共3页
Northern Horticulture
关键词
甜瓜
子叶
组织培养
melon
cotyledon
tissue culture
