摘要
为了探索影响桤叶唐棣初代培养无菌苗建立的因素,现以带芽茎段为外植体,研究了不同消毒方式的消毒效果以及基本培养基类型和激素组合对初代培养的影响。结果表明:10%次氯酸钠10 min和0.1%升汞2 min混合使用的消毒方式,取得了较好的消毒效果,污染率和致死率均较低,分别为11.2%和9.5%;MS培养基为较适宜的初代培养,其增殖芽数、茎芽长分别为2.45和1.18 cm;外植体在MS+IAA 0.1 mg/L+6-BA 2.0 mg/L上生长最好。
Stems with buds were used as explants to study how disinfection methods,basic medium and different hormone affect the primary culture of Amelanchier alnifolia Nutt.The results showed that the best disinfection method was using 10% NaClO for 10 minutes and then 0.1%HgCl2 for 2 minutes,it had lower contaminated rate and death rate;MS medium was the best one for primary culture with high multiplication and length of buds,2.45 and 1.18 cm respectively;explants grew well in the medium of MS with IAA 0.1 mg/L and 6-BA 2.0 mg/L.
出处
《北方园艺》
CAS
北大核心
2010年第20期143-145,共3页
Northern Horticulture
关键词
桤叶唐棣
初代培养
影响因素
Amelanchier alnifolia Nutt
initial culture
influencing factors
