侧扁软柳珊瑚(Subergorgia suberosa)乙醇提取物抗氧化和抑菌活性研究

Anti-oxidation and bacteriostasis activity of the ethanol extract of Subergorgia suberosa

对中国南海侧扁软柳珊瑚(Subergorgia suberosa)乙醇提取物及其石油醚相、乙酸乙酯相、正丁醇相进行了抗氧化和抑菌两种生物活性研究。结果表明:中国南海侧扁软柳珊瑚乙醇提取物及其各萃取相对羟自由基的清除效果好,在50mg/L时清除效率均高于同浓度的合成抗氧化剂TBHQ和BHT;石油醚相、乙酸乙酯相对DPPH自由基和超氧阴离子自由基的清除效率优于正丁醇相和乙醇提取物。另外,对5种海洋细菌、4种陆地细菌进行了抑菌活性测定,发现乙醇提取物及其各萃取相对海洋细菌的最低抑菌浓度(MIC)为100g/L左右,对受试陆地细菌未见抑菌作用。

Ethanol extract from Subergorgia suberosa from the South Sea of China was obtained and was partitioned with petroleum ether,ethyl acetate,and n-butanol.The ethanol extract and the three organic phases showed better ·OH-scavenging efficiency than the synthetic antioxidants,TBHQ and BHT,at 50 mg/L.The scavenging efficiencies of the petroleum ether phase and the ethyl acetate phase to DPPH· and O 2^—· were higher than those of the ethanol extract and the n-butanol phase,respectively.Furthermore,for all samples the bacteriostasis activities against five marine bacteria and four terrestrial bacteria were also detenmined.All samples＇ MICs against the five marine bacteria were about 100mg/ml and,no bacteriostasis activity was found against the terrestrial bacteria tested.