摘要
以杜仲（ＥｕｃｏｍｍｉａｕｌｍｏｉｄｅｓＯｌｉｖ．）幼苗的茎、叶为外植体，在附加植物激素的ＭＳ、Ｂ５和Ｈ培养基上，诱导出了大量的愈伤组织（平均诱导率９４．２％）。在继代培养中建立了３个愈伤组织无性系。绝大多数愈伤组织系以橡胶烃表示的杜仲胶含量都超过了对照（幼苗茎、叶）的５０％。Ｂ５培养基适宜于生产杜仲胶，附加激素Ｂ有利于提高含胶量。愈伤组织系的平均含胶量５．６２％，为对照的５９．４％。
The stem and leaf of the seedlings of Eucommia
ulmoides Oliv.being used as explants ,a large amount of callus was induced on MS,B5 and H
medium supplemented with plant hormones with an average induction frequency of 94.2%
.Three callus clones were established in the course of the subculture of callus.Gutta content
that was expressed as rubber-hydrocarbon content exceeded the control's (the stem and leaf of
seedlings)by 50% for the overwhelming majority of callus clones.B5 medium was suitable for
the production of gutta and hormone B was advantageous to the increase of gutta content.An
average gutta content of the callus clones was 5.62% or 59.4% of the control's.
出处
《特产研究》
1999年第1期1-5,共5页
Special Wild Economic Animal and Plant Research
基金
吉林省科委资助
关键词
杜仲
组织培养
杜仲胶
Eucommia ulmoides Oliv.
Tissue culture
Gutta
