接种联合固氮菌对毛竹实生苗生长的影响

Effects of Inoculating Associated Nitrogen Fixing Bacteria to Moso Bamboo Seedlings Growth

将从毛竹根际分离的２＃菌株（多粘芽孢杆菌），１２＃、１４＃菌株（地衣芽孢杆菌）和从浙江淡竹根际分离的７＃菌株（未最后确定种名），分别培养制成菌悬液，回接毛竹实生苗，进行人工控制条件下的无菌盆栽（沙培）试验。结果显示接菌能不同程度地提高毛竹幼苗成活率并促进其生长，尤其是对根鲜质量增加明显，增幅为４４．１％～７６．９％，分别达到显著（１４＃菌）和极显著（２＃、１２＃、７＃菌）水平。同时，接种联合固氮菌明显提高苗木体内的氮素含量，植株含氮量增幅达２２％～３８．１％。另外，统计分析表明：在无土著菌生长和竞争的条件下，幼苗期接菌处理比种子发芽期接菌处理具更显著的促进植株生长和提高植株含氮量的效果。

Four strains, 2 # (Bacillus polymyxa), 12 #,14 # (B. licheniformis) , isolated from Phyllostachys heterocycla var. pubescens rhizosphere, 7 #(The species name have not been determined finally),isolated from P. meyeri rhizosphere were cultured and prepared into liquid inoculums and inoculated back to moso bamboo (Phyllostachys heterocycla var. pubescens) seedlings.Potted culture (sand culture) under artificial and sterile conditions was then conducted. The results showed that all of four strains were,more or less, able to raise the survival rate and promote the seedling growth in various aspects. Especially for root fresh weight were increased by 44.1%～76.9%,reaching significant level (14 #) and remarkable significant level (2 #,12 #,7 #) respectively.The nitrogen content of seedlings was also increased considerably,increase rate reach to 22%～38%. Meanwhile,statistical analysis results showed, under the condition of no indigenous bacteria, inoculating stage will also influence the growth of seedling,when inoculated on seedling stage the seedlings’ growth and total nitrogen content was better and higher than that inoculated on seed germinating stage,the diversity reach to remarkable significant level.