杨尺蠖核型多角体病毒增殖技术的研究

A Studies on the Technology of Propagation of the Nuclear Polyhedrosis Virus of the Poplar Looper, Apocheima cinerarius

昆虫病毒是一种很好的生物杀虫剂，其特异性强，致病力高，安全、不污染环境。目前国外已有３０多种昆虫病毒杀虫剂进行了登记、注册、生产和应用［１］。然而如何解决病毒的增殖技术和降低生产成本，是病毒商品化生产的重要问题。虽然细胞大规模培养生产病毒杀虫剂的研究...

This paper deals with the study on the main factors,such as the infection concentration,infection instar and temperature,affecting the propagation of the Nuclear Polyhedrosis Virus (NPV) of poplar looper, Apocheima cinerarius, the artificially reared 3～4 instars larvae were fed with the surface inoculated medium and it proved that the optimal concentration is 2.5×10 6 PIB·mL -1 on condition that the temperature is 20 ℃.The result showed that the 4 instar larvae were the best for inoculation.The average propagating total of polyhedral inclusion bodies (PIB) is 2.40×10 9～2.43×10 9 PIB.