中国东海冈村凹顶藻 Laurencia okamurai 中的倍半萜类成分（英文）

Sesquiterpenes from Chinese Red Alga Laurencia okamurai

目的:对采自中国东海的红藻冈村凹顶藻 Laurencia okamurai 的化学成分进行研究, 从中寻找具有生物活性的次生代谢产物。方法:运用硅胶柱层析和凝胶柱层析对冈村凹顶藻的乙醚提取物进行分离纯化; 根据其理化性质, 结合现代波谱分析技术(MS, NMR 等)并与文献报道的化合物数据相比较, 对分离得到的化合物进行结构鉴定。结果:从冈村凹顶藻中分离并鉴定了 9 个倍半萜类化合物, 分别为 laurokamurene C (1), debromoaplysinol (2), aplysinol acetate (3), debromoisolaurinterol (4), iso-laurinterol (5), filiformin (6), α-isobromocuparene (7), cuparene-type ether (8) 和 deoxyprepacifenol (9)。结论:化合物 1 为新化合物, 是天然界中发现的第三个重排的月桂烷型倍半萜, 3 系首次从天然界分离得到, 化合物 2-9 对真菌 Cladosporium cucumerinum 均无明显的抑制活性。

AIM： To study chemical constituents of red alga Laurencia okamurai Yamada to search new bioactive substances. METHODS： The Et2O extract of Laurencia okamurai was subjected to repeated column chromatography on Sephadex LH-20 and silica gel to afford nine pure compounds （1-9）, whose structures were elucidated on the basis of spectroscopic analysis and by com- parison with the data reported in the literature. RESULTS： Nine sesquiterpenes were isolated and identified as laurokamurene C （1）, debromoaplysinol （2）, aplysinol acetate （3）, debromoisolaurinterol （4）, isolaurinterol （5）, filiformin （6）, α-isobromocuparene （7）, cuparene-type ether （8） and deoxyprepacifenol （9）, respectively. CONCLUSIONS： Compound 1, a new sesquiterpene, represents the third example with a novel rearranged laurane-type skeleton, exceptionally prone to decompose at room temperature. Compound 3 was shown for the first time to be true natural product. In antifungal bioassay, compounds 2-9 were found to be inactive against the fungus Cladosporium cucumerinurn.