半夏厚朴汤君药配伍抗抑郁效应研究（英文）

Antidepressant-like Effects of Monarch Drug Compatibility in Banxia Houpu Decoction

目的:研究半夏厚朴汤君药半夏与臣药厚朴、佐药紫苏叶和使药生姜等配伍抗抑郁协同作用。方法:通过小鼠强迫游泳实验评价厚朴酚与和厚朴酚混合物(HMM)、厚朴多糖(PMB)、生姜油(OGR)、生姜多糖(PGR)、紫苏油(OFP)、紫苏多糖(PFP)对半夏总碱(ARP)、半夏多糖(PRP)改善游泳行为和单胺类神经递质异常的作用。结果:在所有配伍组中, 未改变游泳不动时间的无效剂量 PRP 与有效剂量 HMM 配伍后最有效, 并产生显著的协同效应。另外, PRP 和 OFP 配伍也显示出一定的协同效应。这二种配伍均能提高强迫游泳小鼠额叶皮层 5-羟色胺(5-HT)及多巴胺(DA)水平。结论:半夏厚朴汤中臣药、佐药可通过逆转 FST 小鼠 5-HT 能及 DA 能系统异常增强君药抗抑郁作用, 并在减少 FST 小鼠游泳不动时间方面显示出协同效应。而使药对君药无相互作用。

AIM： To investigate the possible synergistic antidepressant-like actions of rhizome pinelliae （monarch drug） with magnolia bark （minister drug）, folium perillae （guide drug）, ginger rhizome （assistant drug） in Banxia Houpu decoction. METHODS： The influences of mixture of honokiol and magnolol （HMM）, polysaccharides （PMB） from magnolia bark, essential oil （OGR） and polysaccharides （PGR） from ginger rhizome, essential oil （OFP） and polysaccharides （PFP） from folium perillae on the ability of total alkaloids （ARP） and polysaccharides （PRP） from rhizome pinelliae to attenuate abnormalities of behaviors and neurotrausmitters using the forced swimming test （FST） in mice. RESULTS： Among compatibility groups, the ineffective dose of PRP altered swimming immobility time when given in combination with an effective dose of HMM. This combination was the most effective and led to a synergistic effect. In addition, combination of PRP with OFP also produced a synergistic effect. These two combinations increased serotonin （5-HT） and dopamine （DA） levels simultaneously in prefrontal cortex in the mouse FST. CONCLUSION： The minister and guide drugs enhanced the monarch drug to exert antidepressant-like actions by attenuating abnormalities in the serotonergic and dopaminergic systems, resulting in a synergistic effect on immobility reduction in the FST. However, the assistant drugs showed no interaction to the monarch drug.