原肌球蛋白1在非小细胞肺癌中的表达及意义

Expression and significance of tropomyosin 1 in non-small cell lung cancer

目的研究原肌球蛋白1(TM1)在非小细胞肺癌(NSCLC)中的表达及其临床意义。方法以15例正常肺组织为对照,采用RT-PCR、免疫印迹及免疫组化方法检测17例腺癌和15例鳞癌及其癌旁组织中TM1表达。结果在mRNA水平,肺癌、癌旁及正常组织中TM1的表达无统计学差异。在蛋白水平,15例对照组织中,TM1呈高水平表达,而32例NSCLC癌组织中TM1蛋白呈低水平表达,癌旁组织中TM1表达介于两者之间(P<0.01)。在不同的临床分期间,肿瘤组织中TM1表达无统计学差异。结论 TM1蛋白低表达可能与NSCLC的发生有关,有望成为NSCLC诊断与治疗的新靶点。

Objective To investigate the expression and significance of tropomyosin 1（TM1） in patients with non-small cell lung cancer（NSCLC）.Methods RT-PCR,Western blot and immunohistochemistry were applied to detect the expression levels of TM1 in cancer tissues and tumor-adjacent lung tissues of 32 patients with NSCLC and normal lung tissues of 15 patients with chest trauma or inflammation.Results The expressions of TM1 mRNA were comparable among cancer tissues,tumor-adjacent lung tissues and normal lung tissues. However,TM1 protein expression was significantly higher in the normal lung tissues（P0.01）,which was lower in cancer tissues and was in between in tumor-adjacent lung tissues. The expression levels of TM1 in different stages of NSCLC were not significantly different.Conclusion The low-expression of TM1 protein may play an important role in pathogenesis of human NSCL,which could become a potential therapeutic target for NSCLC.