混合淋巴细胞反应体系中胎盘间充质干细胞的免疫抑制效应

Immunosuppressive action of human placenta mesenchymal stem cells in mixed lymphocyte reaction system

背景:胎盘由滋养细胞及大量起源于胚外中胚层的间充质和血管共同组成,提示胎盘中存在间充质干细胞成分。目的:探索胎盘间充质干细胞对混合淋巴细胞反应体系中淋巴细胞增殖和细胞因子分泌的影响,评价其免疫学特性。方法:分离培养胎盘间充质干细胞,采用人以及兔双向混合淋巴细胞反应体系,根据胎盘间充质干细胞:外周血单个核细胞的不同比例(1:5,1:10,1:20,1:50,1:100,1:500)加入丝裂霉素处理胎盘间充质干细胞。3H掺入法测定淋巴细胞增殖率并用ELISA法测定混合培养上清液中白细胞介素2和γ-干扰素的含量。结果与结论:胎盘间充质干细胞抑制人或兔混合淋巴细胞反应体系中淋巴细胞的增殖,抑制率与加入的胎盘间充质干细胞数量成正比,同时胎盘间充质干细胞减少混合淋巴细胞反应中细胞因子白细胞介素2、γ-干扰素的分泌。提示胎盘间充质干细胞具有免疫调节作用,可以抑制同种异体或异种混合淋巴细胞反应体系中淋巴细胞的增殖。

BACKGROUND：Placenta comprises trophoblasts, blood vessels and plenty of mesenchyme that derived from the mesoderm.Thus, there are mesenchymal stem cells （MSCs） in placenta.OBJECTIVE：To explore the effect of placenta MSCs （PMSCs） on the proliferation of T lymphocytes and secretion of cytokines in the mixed lymphocyte reaction system, and to evaluate the immunological characteristics of PMSCs.METHODS：The two-way mixed lymphocyte reaction （MLR） system both for human and rabbits were established.After adding PMSCs which was treated by the mitomycin with different ratios （1：5, 1：10, 1：20, 1：50, 1：100, 1：500） in the MLR system, the lymphocyte proliferation rate were measured by 3H incorporation, and the content of interleukin-2 and interferon-γ in the supernatant was detected by ELISA.RESULTS AND CONCLUSION：The proliferation of T lymphocytes could be inhibited to different extents by adding different ratios of PMSCs to human or rabbit PBMCs, and the inhibition rate increased as the ratio of PMSCs to PBMCs increased.Meanwhile, PMSCs could reduce cytokines interleukin-2 and interferon-γ secretion in the mixed lymphocyte reaction system.The PMSCs have the immune regulation ability and inhibit xenogeneic immune cells.