骨髓间充质干细胞成骨过程的诱导因素和信号转导调控

Inducement factors and signal transduction regulation in the osteogenic process of bone marrow mesenchymal stem cells

背景:骨髓间充质干细胞在适宜的培养条件下可被诱导分化成各种组织细胞,此外还具有基质细胞的特性,能够分泌各种细胞因子,支持造血功能,是组织重塑和功能修复的理想细胞来源。目的:对影响骨髓间充质干细胞成骨过程的各种因素和涉及的常见信号转导通路进综述。方法:应用计算机检索Pubmed数据库(1998-01/2009-05),以"bone mesenchymal stem cell,osteogenesis"为检索词;应用计算机检索维普数据库(1998-01/2009-05),以"成骨、骨形成蛋白、骨髓间充质干细胞"为检索词。结果与结论:共收集108篇关于骨髓间充质干细胞和骨形成蛋白的文献,中文31篇,英文77篇。排除发表时间较早、实验设计科学性较差的文献,共15篇文献符合标准被纳入。骨髓间充质干细胞其成骨过程在很大程度上取决于诱导分化条件基本辅剂、骨形成蛋白及其他一些细胞因子和激素均在此列,此外多条信号转导通路亦参与调控骨髓间充质干细胞成骨过程热休克蛋白在骨髓间充质干细胞增殖、分化、成组织过程及应对外界刺激的保护效应的信号通路调控机制值得关注。

BACKGROUND：Bone marrow mesenchymal stem cells （BMSCs） can be induced to differentiate into various cells under suitable conditions.In addition, BMSCs, with the characteristics of stromal cells, can secrete various cytokines, support hematopoietic function, and BMSCs are a source of ideal cells for tissue remodeling and function repair.OBJECTIVE：To review various factors that can influence osteogenic process of BMSCs and common signal transduction pathway.METHODS：A computer-based search was performed in PubMed database （1998-01/2009-05） with the key wrds of ＂bone mesenchymal stem cell, osteogenesis＂.Vip database （1998-01/2009-05） was searched with the key wods of ＂osteogenesis, bone morphogenetic protein, bone marrow mesenchymal stem cells＂.RESULTS AND CONCLUSION：A total of 108 articles concerning bone marrow mesenchymal stem cells and bone morphogenetic protein were retrieved, including 31 Chinese articles and 77 English articles.Those published earlier or with poor experimental design and with poor scientific evaluation were excluded, and finally 15 articles were included.Osteogenesis procedure of BMSCs mostly depends on the induced differentiation situation including the basic adjuvants, bone morphogenetic protein, some other cytokines and hormones.In addition, a series of signal pathways plays an important role in the control and interaction of this process.Heat shock protein is worthy of paying attention on affecting the process of proliferation, differentiation and the protective mechanism of signaling adjustment for stimulation outside BMSCs.