佐剂性关节炎模型关节液与血清白细胞介素17及肿瘤坏死因子α表达及青蒿琥酯的干预

Influence of artesunate on synovial fluid and serum expressions of interleukin-17 and tumor necrosis factor-alpha in rabbits with adjuvant arthritis

背景:已证实青蒿琥酯具有抗风湿作用,但其具体作用机制尚不明确。目的:探讨青蒿琥酯对佐剂性关节炎模型兔关节液及血清中白细胞介素17及肿瘤坏死因子α表达的影响。方法:建立佐剂性关节炎实验兔模型,造模诱发第1天,青蒿琥酯组按20mg/(kg·d)灌服青蒿琥酯7d,羟氯喹组按5mg/(kg·d)灌服羟氯喹7d,模型对照组灌服生理盐水3mL/d。另取10只未经干预的大耳白兔作为正常对照组。分别于造模结束时及给药5周后,应用酶联免疫吸附法检测各组关节液及血清中白细胞介素17及肿瘤坏死因子α的水平。结果与结论:青蒿琥酯组、羟氯喹组关节炎症状得到明显改善,关节液及血清中的白细胞介素17、肿瘤坏死因子α质量浓度均明显降低(P<0.05或P<0.01),且青蒿琥酯组降低幅度明显大于羟氯喹组(P<0.05)。提示青蒿琥酯可能通过抑制白细胞介素17及肿瘤坏死因子α的分泌而产生免疫调节作用,其抗炎疗效优于羟氯喹。

BACKGROUND：It has been proved that rheumatoid can be cured by artesunate.But the specific mechanism remains poorly understood.OBJECTIVE：To explore the effects of artesunate on the level of interleukin-17 and tumor necrosis factor-α（TNF-α） in rabbit with adjuvant arthritis.METHODS：The rabbits were prepared for adjuvant arthritis models.From the first day after model preparation,rabbits in the artesunate group were drenched with artesunate（20 mg/kg per day） for 7 days.Hydroxychlorquine group were infused with hydroxychlorquine（5 mg/kg per day） for 7 days.Model control group was infused with normal saline（3 mL/kg per day） for 7 days.Additional 10 normal rabbits served as controls.The enzyme-linked immunosorbent assay（ELISA） was used to detect synovial fluid and serum level of IL-17 and TNF-α at the end of model preparation and 5 weeks after medication.RESULTS AND CONCLUSION：Compared with model rats,the serum and synovial fluid levels of IL-17 and TNF-α in the artesunate group and hydroxychlorquine group were obviously decreased（P〈0.05 or P 0.01）,especially in the Artesunate group,which was greater than that of the hydroxychlorquine group（P〈0.05）.The results revealed that artesunate can down-regulate the serum and synovial fluid level of IL-17 and TNF-α of adjuvant arthritis.Artesunate has better effect than hydroxychlorquine on treatment of inflammation.