丹参素在大鼠主要组织分布中的动力学特征

Pharmacokinetic features of Danshensu in rat main tissues

背景:近年来对丹参素体内药动学的研究已有很多,但丹参素在大鼠主要组织中特别是皮肤和骨组织中的分布动力学研究尚未见全面的报道。目的:研究大鼠静脉注射复方丹参液后丹参素在主要组织中的分布动力学。方法:采用液液萃取法对SD大鼠血清和主要组织样品(肾、脾、心、肝、肺、腹部皮肤、背部皮肤和骨组织),以对羟基苯甲酸为内标,甲醇-1%冰醋酸(8:92)为流动相,用高效液相色谱法于280nm处检测,液质联用进行定性分析。结果与结论:大鼠静脉注射复方丹参液后,除脑组织外,在血清和其它组织中均检测到丹参素,用所建立的方法绘制了其经时血药浓度曲线和组织浓度柱形图。研究表明大鼠静脉注射复方丹参液后,可以在主要组织中(除脑外)快速分布,其动力学类型属二室模型。

BACKGROUND：The in vivo pharmacokinetic features of Danshensu have been reported,but there are few studies concerning the distribution of Danshensu in rat main tissues.OBJECTIVE：To study the distribution and pharmacokinetics of Danshensu in rat main tissues following intravenous injection of compound Danshen solution.METHODS：Liquid-liquid extraction was used to deal with the serum and main tissues（kidney,spleen,heart,liver,lung,abdomen and back skins,as well as bone tissues）.The conditions for HPLC were：4-hydroxy-benzoic acid served as the internal standard,the CH3OH-1%CH3COOH（8：92） served as mobile phase,and detection wavelength was 280 nm.RESULTS AND CONCLUSION：Except the brains,Danshensu could find in the rat serum and the main tissues after intravenous injection of compound Danshen solution.The column of serum concentration-time and tissues concentration-time was drew using the established methods.The results demonstrated that except the brains,Danshensu could distribute rapidly in main tissues after intravenous injection of compound Danshen solution,and the pharmacokinetics fitted to two-compartment model.