骨碎补总黄酮与柚皮苷干预股骨干骨缺损模型大鼠骨愈合的比较

Effects of drynaria fortunei total flavonoid versus naringin on bone healing in rat femoral shaft bone defect models

背景:骨碎补能增强成骨细胞活性、提高骨密度、维持骨微结构的完整程度。目的:观察中药骨碎补不同成分对于骨愈合的影响。方法:Wistar雄性大鼠30只建立股骨干骨缺损模型,分别用提取的骨碎补成分骨碎补总黄酮及柚皮甙,以及从柑橘皮中提取的橙皮甙按20g/kg对造模大鼠进行灌胃治疗,于治疗21d后取血及骨标本检测大鼠骨愈合程度和血液流变学指标。结果与结论:骨碎补不同成分均对骨愈合有益,且骨碎补中总黄酮效果优于骨碎补中柚皮甙及柑橘皮中的橙皮甙(P<0.05),骨碎补总黄酮可有效抑制低剪切速下大鼠的血液黏度,对红细胞聚集指数具有显著降低作用,并可显著抑制大鼠血小板聚集性,降低血小板黏附性。说明骨碎补可利于骨愈合,其中总黄酮效果更佳,且对大鼠血液流变学有一定影响。

BACKGROUND：Drynaria fortunei can enhance osteoblast activity,elevate bone mineral density,and maintain bone microarchitecture.OBJECTIVE：To observe the effects of different compositions of drynaria fortunei on bone healing.METHODS：Totally 30 male Wistar rats were established for femoral shaft defect models and treated by intragastric administration of drynaria fortune extraction and hesperidin（20 g/kg）.The bone healing and hemorheology indexes were detected at 21 days after treatment.RESULTS AND CONCLUSION：The different compositions of drynaria fortunei were all beneficial to the bone healing,especially total flavonoid,which showed better results than naringin and hesperidin（P〈0.05）.The drynaria fortunei total flavonoid could inhibit effectively the blood viscosity of the rats in speed of lower shearing,and repress notably the index number of aggregation of the red cells.In addition,it also could suppress notably platelet aggregation,and reduce platelet adhesion.The findings demonstrated that,drynaria fortune,especially total flavonoid,can be propitious to the bone healing,which has function to the hemorheology of rats.