肌球蛋白Ⅱ马达活性与肌动蛋白纤维共同调节哺乳动物细胞胞质分裂

Myosin Ⅱ motor activity and actin filament cooperatively regulate cytokinesis of mammalian cells

背景:哺乳动物细胞胞质分裂过程中伴随着一系列细胞骨架改变,骨架蛋白的相互协调对胞质分裂起重要作用。目的:观察细胞骨架抑制剂对胞质分裂的影响。方法:分别利用单一的细胞骨架抑制剂细胞松弛素D、秋水仙素和肌球蛋白Ⅱ马达活性抑制剂blebbistatin及其组合对胞质分裂时期的大鼠肾上皮细胞进行干预。结果与结论:细胞骨架抑制剂对胞质分裂的抑制作用不是叠加的,同时对细胞整体施加细胞松弛素D和blebbistatin不能抑制分裂,而单一施加细胞松弛素D以及在整体blebbistatin作用下单侧极区施加细胞松弛素D均可以抑制细胞胞质分裂进程,说明协调肌动蛋白和肌球蛋白Ⅱ马达活性是完成胞质分裂的关键因素。

BACKGROUND：Cytokinesis of animal cells experiences a series change of cytoskeleton.The interaction of skeleton protein plays a vital role on cytokinesis.OBJECTIVE：To observe the influence of cytoskeleton inhibitor on cytokinesis.METHODS：Normal rat kidney cells in cytokinesis were treated separately with cytoskeleton inhibitors including cytochalasin D（CD）,colchicine（COLC） or blebbistatin（myosin Ⅱ motor activity inhibitor）.RESULTS AND CONCLUSION：It was showed that the roles of cytoskeleton inhibitors to cytokinesis were not superimposed,fordifferent treatment of inhibitors corresponding to different results.Whole region treatment with cytochalasin D and blebbistatin could not cause a fierce inhibition of cytokinesis.While cells which treated with cytochalasin D or treated with CD in the pole region of daughter cells in presence of blebbistatin showed an effective inhibition of cytokinesis.The study indicated that the interaction in actin and the activity of myosin Ⅱ motor is vital to cell shape and operation in every stage of cytokinesis.