骨组织工程中的细胞外基质材料及其种子细胞

Extracellular matrix materials and seeded cells of bone tissue engineering

背景:目前,骨组织工程在骨再造及骨缺损修复领域方面发展迅速,其中细胞外基质材料和种子细胞的选择应用是决定骨组织工程成功的前提和基础。目的:对骨组织工程中的细胞外基质材料和种子细胞的研究现状进行综述,并分析了这些细胞外基质材料和种子细胞的优缺点及今后尚待解决的问题和发展趋势。方法:应用计算机检索Pubmed数据库和CNKI数据库中1994-01/2009-12关于骨组织工程的文章,在标题中和摘要中以"骨组织工程,种子细胞,细胞外基质材料"或"bone tissue engineering,seeded cell,extral cellular matrix materials"为检索词进行检索。选择文章内容与骨组织工程中的种子细胞和细胞外基质材料有关者,同一领域文献则选择近期发表或发表在权威杂志文章。初检得到246篇文献,根据纳入标准选择相关的30篇文献进行综述。结果与结论:细胞外基质材料尚不够完善,还存在这样或那样的缺陷。如基质材料的降解率与成骨速度不协调;基质材料在体内所引发的免疫反应等;种子细胞的来源及培养体系尚未完全确定;种子细胞与细胞外基质材料的相互作用关系,尤其是如何增加种子细胞在基质材料表面的生物活性及分化方向方面,仍需进一步深入研究。

BACKGROUND：With the rapid development of bone tissue engineering in areas of bone reconstruction and repair of bone defects,how to choose and apply ideal seeded cells and extracellular matrix materials determine the success of bone tissue engineering.OBJECTIVE：To summarize the research progress of the extracellular matrix materials and seed cells in bone tissue engineering,and to introduce their merits and shortcomings,as well as the existing problems and developing trends on them in bone tissue engineering.METHODS：Database of CNKI and Pubmed （1994-01/2009-12） were retrieved for articles about bone tissue engineering by screening the Key words ＂bone tissue engineering,seeded cell,extracellular matrix materials＂ in Chinese and in English in the titles and abstracts.Articles associated with extracellular matrix materials and seed cells in bone tissue engineering were included,those published recently or in authority journals were preferred in the same field.There were 246 articles screened out after the initial survey.Then 30 articles were selected according to the inclusive criteria for the review.RESULTS AND CONCLUSION：The extracellular matrix materials still have some flaws,such as the degradation rates are inconsistent with bone formation rate;matrix materials induce immune responses in vivo;the source of seed cells and culture system are not fully established;the interaction between seed cells and extracellular matrix materials,especially how to increase the bioactivities and differentiation of seed cells on the matrix materials.These problems need further investigation.