三种不同表面处理的种植体对成骨细胞增殖的比较研究

Study on the Proliferation of Osteoblasts in 3 Different Implant Surfaces In Vitro

目的:探讨钛合金表面3种生物陶瓷涂层对成骨细胞黏附、生长、增殖的影响。方法:在钛合金(TLM)基体表面进行喷砂酸蚀和微弧氧化(MAO)技术处理,制备出喷砂酸蚀(SLA)、载银(MAO-Ag)和非载银(MAO)3种不同材料的表面生物陶瓷涂层;扫描电镜(SEM)和X射线能谱(EDX)仪观察分析膜层的表面形貌及元素构成;观察不同材料表面成骨细胞生长状况,通过碱性磷酸酶(ALP)活性测定法和四氮唑盐(MTT)法检测不同材料对细胞增殖的影响。结果:微弧氧化制备的涂层表面多孔,能谱分析证实MAO-Ag涂层表面含有银元素;SLA、MAO和MAO-Ag 3种材料均能促进成骨细胞的增殖,并随着时间延长,ALP含量及吸光度(A)值均逐渐升高。MAO组优于SLA组,MAO-Ag组优于MAO组和SLA组,均具有统计学意义。结论:3种表面生物陶瓷涂层均能促进成骨细胞的增殖,MAO-Ag组效果最佳。

Objective： To evaluate cell coating, growth and proliferation of osteoblasts on three kinds of bio-ceramic coatings for implant surfaces. Methods： SLA, non-silver（MAO） and silver（MAO-Ag） coatings were prepared by machine grinding and microarc oxidation skill on TLM titanium alloy （Ti-3Zr-2Sn-3Mo-25Nb）. Surface chemistry and morphology / pore characteristics were characterized with SEM and EDX. Osteoblasts proliferation were measured by ALP activities and MTT assay. Results： Porous ceramic coatings were prepared by MAO and Ag peak were detected on MAO-Ag coating by EDX. SLA, MAO and MAO-Ag coatings all found to promote osteoblast growth. ALP content and OD values were increased gradually. MAO coating was better than the SLA coating, and MAO-Ag coating was better than MAO and SLA coatings （P〈0.05）. Conclusion： All three coatings show good biocompatibility with osteoblasts and can promote the proliferation of osteoblasts, MAO-Ag coating is the best.