骨延长速度对成骨过程的影响

Influence of bone elongation speed on osteogenesis

背景:骨延长过程的影响因素很多,到目前为止仍有好多问题尚未明确。延长速度是影响骨延长进程的一个重要因素,对其进一步研究将会有助于对骨延长过程的理解。目的:观察延长速度对骨延长成骨作用的影响。方法:在大鼠左侧股骨中部截骨后,安装单边外固定架予以固定。从术后第7天开始,对2组动物分别进行为期2周速度为0.5mm/d或1mm/d的骨延长。于术后第21天将动物处死获得标本,采用影像学、骨密度测定、延长部类骨组织硬度测定及组织学评价来定性、定量检测新生骨组织。结果与结论:术后第21天,1mm/d延长组大鼠的骨密度和类骨组织硬度虽高于0.5mm/d延长组,但差异无显著性意义。组织学表现上,0.5mm/d延长组中见到螺旋状排列的纤维束,但在1mm/d延长组中则基本消失,变为近似直行的纤维束。两组均未见到骨不连或过早骨化等并发症。提示采用1mm/d的延长速度,会加快骨延长,缩短治疗时间,不会影响成骨过程而导致治疗失败。

BACKGROUND：Bone elongation is a complicated process that remains poorly understood. Elongation speed is a major factor that affects the bone consolidation. Further research could provide more information and help understand bone lengthening. OBJECTIVE：To investigate the influence of elongation speed on distraction osteogenesis. METHODS：Following osteotomy at the middle segment of rat left femur,the external frisket was installed for fixation. The lengthening protocol included a seven-day latency period,followed by an incremental lengthening of 0.5 mm/d or 1 mm/d for 2 weeks. The animals were sacrificed 21 days after the operation. Bone hardness and bone mineral density was determined. Histological examinations were also performed. RESULTS AND CONCLUSION：No statistical difference was observed in bone mineral density and hardness between the 0.5 mm group and the 1 mm group on 21 days after the operation. Helix fibers were observed in 0. 5 mm/d group,while more straight fibers in 1 mm/d group were detected. No complication was observed in both groups. Results indicated that 1 mm/d elongation can accelerate the procedure,shorten the treatment time without further complications.