ZBTB20基因敲除小鼠出生后肝脏甲胎蛋白基因持续高表达的病理学意义

Pathological implication of postnatally up-regulated AFP expression in ZBTB20 knockout neonatal mice

目的探讨出生后肝脏甲胎蛋白(AFP)基因异常高表达在ZBTB20基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型中可能的病理作用。方法将ZBTB20单基因敲除小鼠与AFP单基因敲除小鼠杂交,建立ZBTB20与AFP的联合基因敲除小鼠模型,观察并监测其与ZBTB20单基因敲除小鼠在生长发育、存活率及血糖水平上的差异。结果 ZBTB20/AFP双基因敲除小鼠繁殖成功后,通过观察和监测发现其在生长发育、生存率及血糖代谢等方面与ZBTB20单基因敲除小鼠相比无明显差异。结论 ZBTB20单基因敲除小鼠生长发育障碍、严重低血糖和未成年死亡等表型与其AFP基因出生后异常开放无显著关系。

Objective To investigate the pathological roles of postnatally up-regulated alpha-fetoprotein（AFP） expression in ZBTB20knockout mice in postnatal growth retardation,metabolic dysfunction,and pre-mature mortality. Methods ZBTB20and AFP knockout mice were generated by crossbreeding ZBTB20knockout mice with AFP knockout mice. The postnatal growth,survival rate,and blood glucose of ZBTB20/AFP double knockout mice were observed and compared with those of ZBTB20knockout mice.Results There were no significant differences in postnatal growth,survival,and glucose homeostasis between ZBTB20/AFP doube knockout mice and ZBTB20knockout mice.Conclusion The postnatally up-regulated liver AFP in ZBTB20knockout mice is not associated with the growth retardation,hypoglycemia,or pre-mature mortality.