糖肾汤对糖尿病大鼠肾保护作用的实验研究

Protective effect of Tangshentang on kidney in diabetic rats

目的研究糖肾汤对糖尿病大鼠肾保护作用。方法采用四氧嘧啶造成实验性糖尿病大鼠模型,随机分为模型组、糖肾汤治疗组、卡托普利对照组,连续灌胃给药4周,观察治疗前后血糖变化,尿微量白蛋白的排泄,尿α1-微球蛋白水平,血液流变学变化,肾脏组织形态学改变及肾内血流的彩色多普勒超声(color Doppler flow ima-ging,CDFI)血流参数的变化。结果糖肾汤降低了糖尿病大鼠肾重与体重比值,并可使糖尿病模型大鼠尿微量白蛋白排泄量及尿α1-微球蛋白含量明显减少,改善糖尿病模型大鼠血流变异常,减轻/延缓光镜下肾脏结构损害,控制和纠正糖尿病模型大鼠肾血管阻力增高,改善其肾内血流灌注的状况。结论糖肾汤具有延缓实验性糖尿病肾病的作用,明显改善早期糖尿病肾病肾内血流状况,控制肾小球滤过率的改变。

Objective To research the protective effect of the traditional Chinese drug Tangshentang（TST） on kidney in diabetic rats.Methods The diabetic rats were duplicated by alloxan,and randomly divided into 3 groups,which were the diabetic group,the TST group and the captopril control group.The three groups were treated with continuous stomach perfusion for 4 weeks,during this period,changes of blood glucose,excretion of urinary small protein,level of α1-MG,changes of hemorheology,renal histomorphology and blood flow parameters of color Doppler flow imaging（CDFI） were observed.Results TST reduced the diabetic rat＇s ratio of kidney weight and body weight,decreased the excretion of urine microalbumin and content of urine α1-microgluobulins of diabetic rat obviously,improved the abnormal state of the blood rheology,abated and slowed down kidney structural lesion under optical microscope,controlled and modified the increase of renal vascular resistance of rat models,improved the state of blood perfusion.Conclusion TST has delaying effect for experimental diabetic nephrosis by significantly improving the state of blood flow of the pristine diabetic nephropathy and controlling the change of glomerular filtration rate.