大动脉炎患者血管内皮损伤的机制研究

Mechanism of vascular endothelial injury in patients with Takayasu's arteritis

目的探讨血管内皮损伤与大动脉炎活动性的关系,以及在大动脉炎发病机制中的作用。方法选择大动脉炎患者60例,其中活动期40例,非活动期20例,同时选择20例健康人作为对照组。用放免法测定大动脉炎患者血浆内皮素-1(endothelin-1,ET-1)、血管紧张素Ⅱ(angiotensinⅡ,AngⅡ),用双抗体夹心ELISA法检测血管内皮生长因子(vascular endothelial growth factor,VEGF)水平,并检测外周血循环内皮细胞(circulating endothelial cell,CEC)水平。结果大动脉炎活动期患者血浆VEGF、ET-1、AngⅡ和CEC显著高于非活动期患者(P<0.05),非活动期组显著高于正常对照组(P<0.05)。结论大动脉炎患者存在血管内皮的损害,VEGF、ET-1、AngⅡ和CEC参与了TA血管损害的进程,其水平及数量可作为评价大动脉炎活动的临床指标。

Objective To approach the endothelial injury mechanism in patients with Takayasu＇s arteritis and relation to the phases of disease.Methods Sixty patients with Takayasu＇s arteritis were recruited including 40 in active phase and 20 in inactive phase,and 20 healthy persons as control group.The plasma levels of ET-1 and AngⅡwere measured by radioimmunoassay.The plasma level of VEGF was measured by double antibody ELISA.Endothelial cell counts（CEC） were also detected.Results The plasma levels of VEGF、ET-1、AngⅡand CEC in active phase were significantly higher than in inactive phase（P0.05）,inactive phase were significantly higher than in control group（P0.05）.Conclusion Takayasu＇s arteritis patients have endothelial injury.The VEGF,ET-1,AngⅡand CEC take part in progress in vessel injury and may be the marker to evaluate the activity of this disease.