蜕皮激素诱导下家蚕CYP3基因家族的表达变化

Changes in the expression of the CYP3 family genes under the induction of ecdysone in Bombyx mori

为了研究家蚕Bombyxmori CYP3家族基因经蜕皮激素诱导后的表达变化,用蜕皮激素溶液(2×10-3μg/μL)浸泡的桑叶喂食家蚕B.mori5龄幼虫,以不用蜕皮激素处理的桑叶喂食家蚕为对照,采用双跟踪标定定量PCR(dual-spike-in qPCR)方法,检测在蜕皮激素诱导下家蚕中肠和脂肪体内CYP3基因家族的转录水平。结果表明:与对照相比,在蜕皮激素诱导下家蚕幼虫体内脂肪体中CYP302,CYP306和CYP339的转录水平分别上升了191.4,7.4和421倍,在中肠中变化不显著;其余基因转录水平变化不明显或检测不到转录活性。结果说明CYP339基因有可能参与家蚕蜕皮激素的代谢,这为进一步研究P450基因与内源物质的关系提供理论基础。

To study the change in expression of the CYP3 family genes induced by ecdysone,we measured their transcription level by dual spike-in qPCR in the midgut and fat body of Bombyx mori 5th instar larvae fed on mulberry leaves soaked in ecdysone solution（2×10-3μg/μL）with those fed on mulberry leaves not soaked in ecdysone solution as the control.The results showed that compared with the control,the transcription level of CYP302,CYP306 and CYP339 increased by 191.4,7.4 and 421 times in fat body,respectively,and that of the other genes induced by ecdysone did not change significantly or was not detectable.These results suggest that CYP339 gene may be involved in the metabolism of silkworm ecdysone.This work laid a theoretical foundation for further study on the relationship between P450 genes and the metabolism of endogenous substances.