中国桃蛀螟不同地理种群的遗传多样性

Genetic diversity of Conogethes punctiferalis(Guenée)(Lepidoptera:Crambidae)populations from different geographic regions in China

本文应用ISSR分子标记技术对中国桃蛀螟Conogethes punctiferalis11个地理种群的基因组DNA进行了遗传多样性和遗传分化分析。从34条引物中筛选出6条用于ISSR多态性分析,共扩增出211条带,其中209条具多态性,总的多态性条带百分率为99.05%。11个种群的遗传距离在0.0059-0.0237之间,Nei氏基因多样性指数为0.1750,Shannon信息指数为0.2966,遗传分化系数为0.053,基因流高达8.8724。结果提示中国桃蛀螟地理种群因基因流水平较高而种群遗传分化水平保持较低。

Genetic diversity among eleven geographic populations of Conogethes punctiferalis in China was investigated using Inter-Simple Sequence Repeat（ISSR）markers in this study.Six of the 34 used primers were chosen for our ISSR polymorphic analysis.The results showed that 209 bands were polymorphic,making up 99.05% of the total 211 amplified bands.The genetic distances between different C.punctiferalis populations were 0.0059-0.0237.The Nei＇s index,Shannon information index,and coefficient of genetic（gene）differentiation among populations（Gst）was 0.1750,0.2966 and 0.053,respectively,and the estimated value of gene flow from Gst was 8.8724.The results suggest that C.punctiferalis populations in China keep a low level of population genetic differentiation due to a considerable gene flow.