胺鲜酯对高产夏玉米产量及叶片光合羧化酶和保护酶活性的影响

Effects of DA-6 on leaf photosynthetic carboxylase and protective enzyme activities and grain yield of high-yielding summer maize

采用田间小区试验,以高产玉米新品种登海661为材料,研究了拔节期叶面喷施10、20和40 mg.L-1的胺鲜酯(DA-6)对玉米叶片光合羧化酶、保护酶活性和产量的影响.结果表明:喷施胺鲜酯各处理玉米分别比对照(含有表面活性剂和水)增产10.0%(10 mg.L-1)、8.9%(20 mg.L-1)和9.4%(40 mg.L-1),增产效果显著,但各浓度间差异不显著.胺鲜酯处理后,花后玉米的叶面积指数、光合速率、RuBP羧化酶和PEP羧化酶活性均显著上升(P<0.05),且对光合速率、RuBP羧化酶和PEP羧化酶活性的影响随着处理浓度的增加而提高;与对照相比,胺鲜酯处理后吐丝期、灌浆期、乳熟期和蜡熟期叶片SOD、CAT、POD和GSTs活性及可溶性蛋白质含量显著提高(P<0.05),MDA含量显著降低(P<0.05),其中CAT活性随着处理浓度的增加呈上升趋势,其余生理指标各浓度间无显著性差异.

A field plot experiment was conducted to study the effects of foliar spraying DA-6 at the rates of 10,20,and 40 mg·L^-1 at jointing stage on the leaf photosynthetic carboxylase and protec-tive enzyme activities and grain yield of high-yielding summer maize cultivar Denghai 661.Comparing with the control（foliar spraying surfactant and water）,spraying 10,20,and 40 mg·L^-1 of DA-6 increased the grain yield of Denghai 661 significantly,with the increment being 10.0%,8.9%,and 9.4%,respectively,but no significant differences were observed among the DA-6 treatments.Different concentration DA-6 increased the leaf area index,net photosynthetic rate,and RuBPCase and PEPCase activities significantly,and the promotion effects on the net photosynthetic rate and RuBPCase and PEPCase activities increased with increasing concentration of DA-6.After treated with DA-6,the leaf superoxide dismutase,catalase,peroxidase,and gultathione S-transfer-ase activities and the leaf soluble protein content at the stages of silking,grain-filling,milky,and wax maturity all increased significantly,and the leaf malondialdehyde decreased significantly,com-pared with the control.The catalase activity increased with increasing DA-6 concentration,but the other indices had no significant differences among the DA-6 treatments.