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丙酮丁醇梭菌的遗传操作系统 被引量：3

Genetic modification systems for Clostridium acetobutylicum
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摘要 丙酮丁醇梭菌是极具潜力的替代燃料——生物丁醇的合成菌,受到各国研究者的普遍关注。丙酮丁醇梭菌菌株改造是生物丁醇产业化进程中的一项重要工作,其中遗传操作是核心内容之一。以下对丙酮丁醇梭菌的遗传操作系统的发展历史、种类和原理进行了综述,分析了目前几种遗传操作系统的局限性,并对其发展进行了展望。 Clostridium acetobutylicum, a biofuel-butanol producer, has attracted worldwide interests. Strain improvement is important for the process of biobutanol industrialization where efficient genetic modification systems are essential. In this review, the history of genetic modification systems of C. acetobutylicum was introduced, and the types and principles of these systems and their disadvantages are summarized and analysed. The development of updated genetic modification systems for C. acetobutylicum is also proposed.
作者 董红军 张延平 李寅
机构地区 中国科学院微生物研究所
出处 《生物工程学报》 CAS CSCD 北大核心 2010年第10期1372-1378,共7页 Chinese Journal of Biotechnology
基金 国家高技术研究发展计划(863计划)(No2006AA02Z237)资助~~
关键词 丙酮丁醇梭菌 丁醇 菌株改造 遗传操作系统 Clostridium acetobutylicum butanol strain improvement genetic modification systems
分类号 Q78 [生物学—分子生物学]
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参考文献1

二级参考文献10

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共引文献24

同被引文献67

  • 1Zhu L, Dong H, Zhang Y, et al. Engineering the robustness of Clostridium acetobutylicum by introducing glutathione biosynthetic capability. Metab Eng, 2011, 13(4) :426-434.
  • 2Keiski V G, Pakkil. J, Ojamo H, et al. Challenges in biobutanol production: How to improve the efficiency? Renewable and Sustainable Energy Reviews, 2011, 15 ( 2 ) :964-980.
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  • 8Alsaker K V, Paredes C, Papoutsakis E T. Metabolite stress and tolerance in the production of biofuels and chemicals: gene- expression-based systems analysis of butanol, butyrate, and acetate stresses in the anaerobe Clostridium acetobutylicum. Biotechnol Bioeng, 2010, 105 (6) : 1131-1147.
  • 9Baek K T, Vegge C S, Skorko-Glonek J, et al. Different contributions of HtrA protease and chaperone activities to Campylobacter jejuni stress tolerance and physiology. Appl Environ Microbiol, 2011, 77( 1 ) :57-66.
  • 10Tomas C A, Welker N E, Papoatsakis E T. Overexpression of groESL in Clostridium acetobutylicum results in increased solvent production and tolerance, prolonged metabolism, and changes in the cell. transcriptional program. Appl Environ Microbiol, 2003, 69(8) :4951-4965.

引证文献3

二级引证文献12

生物工程学报
2010年 第10期

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