高效液相色谱法评价中药石斛的质量

Quality evaluation of Dendrobii Caulis by HPLC

以联苄类成分杓唇石斛素和石斛酚为指标成分,建立了采用HPLC进行石斛含量测定的方法。采用反相C18色谱柱,以乙腈-0.01%三氟乙酸为流动相进行梯度洗脱。结果表明目标化合物的质量浓度分别在2.29-1 144.00μg/mL之间和1.79-1 196.00μg/mL之间呈线性,R2均大于0.999 7;平均加样回收率分别为102.7%和99.1%。通过对18种不同来源石斛及18批商品石斛的含量测定,表明所建方法灵敏、准确、可靠,可作为中药石斛的质量控制方法。

Bibenzyls are biologically active compounds widely found in Dendrobii Caulis.A HPLC method was developed for the determination of two specific bibenzyls,moscatilin and gigantol.Chromatographic separation was performed on a reverse-phase C18 column employing a binary linear gradient elution with acetonitrile and 0.01% trifluroacetic acid.The linear ranges of moscatilin and gigantol were from 2.29 μg/mL to 1 144.00 μg/mL and 1.79 μg/mL to 1 196.00 μg/mL respectively with good correlation coefficients greater than 0.999 7.The average recoveries measured at three levels were 102.7% and 99.1%,respectively.The validated method was applied for 18 different species of Dendrobium plants and 18 batches of Dendrobii Caulis,which proved to be sensitive,reliable and applicable for the quality control.