23份芥菜型油菜同工酶遗传多样性分析

Genetic Diversity of Twenty-three Brassica juncea Accessions Based on Isozyme Markers

采用酯酶（EST）、淀粉酶（AMY）和过氧化物酶（POD）同工酶电泳,对23份芥菜型油菜材料进行了遗传多样性分析。同工酶电泳共检测到3种酶的多态型条带26条,酯酶和淀粉酶都存在着丰富的多态性。带型分析结果表明,酯酶EST-2、EST-3特征带可能与材料地理来源有关,淀粉酶AMY-2、AMY-3、AMY-4和AMY-5特征带可能与控制籽粒颜色基因的表达有关。参试23份材料的遗传相似系数在0.625~1.0,聚类分析可将其分为冬播型和春播型为主的两大类群,冬播型类群中全部为黑籽材料,春播型类群中包含了所有参试的8个黄籽材料和4个黑籽材料。不同芥菜型油菜品种之间因其类型、籽粒颜色及地区等因素差异而存在较大的遗传差异。

The genetic diversity of 23 Brassica juncea accessions were analyzed by three isozymes,esterase（EST）,amylase（AMY） and peroxidase（POD）.Totally,26 polymorphic isozyme bands were detected among these accessions,and there were rich polymorphisms in the banding patterns of esterase and amylase.The esterase bands EST-2 and EST-3 may be related to geographical origin of the accessions,and,AMY-2,AMY-3,AMY-4 and AMY-5 of amylase to the expressions of genes conferred with seed color.The genetic similarity coefficient of the tested 23 B.juncea accessions ranged from 0.625 to 1.0.All 23 accessions of B.juncea could be divided into two groups,winter sowing and spring sowing group,all accessions in the winter sowing group are black seed,and 8 yellow-seeded and 4 black-seeded accessions are included in the spring sowing group.The results showed that there exists a considerable genetic difference among accessions with different sowing type,seed color and regions.