辣椒抗疫病相关基因的RGA-STS标记的开发

Development of a Sequence Tagged Sites Marker Derived from Resistance Gene Analog (RGA-STS) Associated with Resistance to Phytophthora capsici in Pepper (Capsicum annuum L.)

利用西北农林科技大学园艺学院辣椒课题组克隆的辣椒抗疫病相关的全长基因RGA1(GenBank登录号:GQ386945)设计一对引物,上游引物为BY32,下游引物为RBQC-R。以对辣椒疫病高抗的品种CM334和感病品种EC为试材,利用PCR技术分析辣椒抗疫病的Sequence Tagged Sites(STS)标记。结果表明,在高抗品种CM334中得到700 bp大小的STS700标记,而在感病品种EC中未扩增出相应大小的片段。在多个不同抗疫病辣椒品种中验证说明,STS700标记鉴定辣椒疫病抗性可靠、稳定。

At present study,we developed a pair of primers,upstream primers BY32 and downstream primer RBQC-R with a full-length disease resistance gene analog RGA1（GenBank accession No.GQ386945）,cloned and released by the pepper research group in Northwest AF University.We screened two materials,a high resistant Cv.CM334 and a susceptible Cv.EC,with PCR technology.As a result,we can get a 700 bp marker（STS700） in the highly resistant cultivar CM334,and can not get it in Cv.EC.Furthermore,we checked the transferability of this STS700 marker with several other materials that show different level of resistance to Phytophthora capsici.The results show that the marker developed in this study is stable,reliable in the identification of resistance to Phytophthora capsici.