枯草芽孢杆菌对百合枯萎病的防治效果

Inhibitive Effects of Bacillus subtilis on Liliaceous Fusarium oxysporum

采用菌丝生长速率法及孢子萌发法,研究枯草芽孢杆菌对百合枯萎病菌的抑杀效应。枯草芽孢杆菌无菌滤液在一定体积分数范围内能有效抑制枯萎病菌菌丝生长,减少孢子萌发,且随着无菌滤液体积分数的增加,孢子萌发抑制率也增大。体积分数为20%的枯草芽孢杆菌无菌滤液,处理后7d抑制率可达78.8%。体积分数为60%时,可完全抑制孢子萌发。盆栽防治试验表明,枯草芽孢杆菌菌液对百合枯萎病的发病抑制率达65.3%。且该菌液对百合的生长有一定的促生功效,说明枯草芽孢杆菌对百合枯萎病有抑菌和防病作用,且抑菌作用随枯草芽孢杆菌菌液体积分数的增加而增强。

The inhibitive effects of Bacillus subtilis on liliaceous Fusarium oxysporum was studied by measuring pathogen spore germination and mycelia growth in lab culture dish.The results showed when the volume fraction of Bacillus subtilis bacteria-free filtrate is 20%,7days after treatment,the inhibition rate is 78.8%.Bacteria-free filtrate experiment showed bacteria-free filtrate of Bacillus subtilis can effectively inhibit mycelia growth to a certain extent,reduce the number of germination.When the volume fraction is 60%,the spores can＇t germinate at all.The potted experiment indicates control efficiency of Bacillus subtilis on liliaceous Fusarium oxysporum is 65.3%.And plants treated by Bacillus subtilis grow better than CK.The results indicate Bacillus subtilis has antagonistic effect on liliaceous Fusarium oxysporum,and inhibitive effects are increasing along with the increase of the concentration of Bacillus subtilis.